Volume 28 Issue 6
Nov.  2007
Turn off MathJax
Article Contents
ZHAO Hua-fu, LIU Jia, HU Jian. An Improved Method on Encapsulation Assays by Larval Hemocytes from Ostrinia furnacalis in vitro. Zoological Research, 2007, 28(6): 675-680.
Citation: ZHAO Hua-fu, LIU Jia, HU Jian. An Improved Method on Encapsulation Assays by Larval Hemocytes from Ostrinia furnacalis in vitro. Zoological Research, 2007, 28(6): 675-680.

An Improved Method on Encapsulation Assays by Larval Hemocytes from Ostrinia furnacalis in vitro

  • Received Date: 2007-09-12
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2007-12-22
  • Encapsulation, a kind of cellular immune reaction of insect, plays an important role in eliminating foreign objects such as pathogens and parasites which invade insect hemocoels. As a result of limited observation and complicated manipulations, analysis of encapsulation can not be easily performed in vivo. Encapsulation in vitro resolves these problems to a certain extent. Now, encapsulation assays in vitro are usually performed in 96-well plates in which foreign objects such as sephadex beads and insect hemocytes are contained and observed,but some shortcomings exist in this method. In this paper,an improved analysis method on the encapsulation of Ostrinia furnacalis larvae hemocytes in vitro is introduced. 0.2 mL Eppendorf tubes are the substitute for the 96-well plates and they are fixed to a revolving rotator at a speed of 4 circles per min. The states of hemocytes and sephadex beads in insect’s hemocoels are replicated under this method. Results showed that the states of hemocytes were improved and encapsulation was enhanced under the new method and anticoagulants might inhibit the encapsulation ability of hemocytes.
  • loading
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (1573) PDF downloads(1937) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return