Molecular characterization of an IL-1&beta; gene from the large yellow croaker (Larimichthys crocea) and its effect on fish defense against Vibrio alginolyticus infection

Jun WU; Yu-Hong SHI; Xue-Heng ZHANG; Chang-Hong LI; Ming-Yun LI; Jiong CHEN

Volume 36 Issue 3

May 2015

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Article Navigation > Zoological Research > 2015 > 36(3): 133-141

Jun WU, Yu-Hong SHI, Xue-Heng ZHANG, Chang-Hong LI, Ming-Yun LI, Jiong CHEN. Molecular characterization of an IL-1β gene from the large yellow croaker (Larimichthys crocea) and its effect on fish defense against Vibrio alginolyticus infection. Zoological Research, 2015, 36(3): 133-141.

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Jun WU, Yu-Hong SHI, Xue-Heng ZHANG, Chang-Hong LI, Ming-Yun LI, Jiong CHEN. Molecular characterization of an IL-1β gene from the large yellow croaker (Larimichthys crocea) and its effect on fish defense against Vibrio alginolyticus infection. Zoological Research, 2015, 36(3): 133-141.

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Molecular characterization of an IL-1β gene from the large yellow croaker (Larimichthys crocea) and its effect on fish defense against Vibrio alginolyticus infection

1 Laboratory of Biochemistry and Molecular Biology, School of Marine Sciences, Ningbo University, Ningbo 315211, China;
2 Donghai Sea Collaborative Innovation Center for Industrial Upgrading Mariculture, Ningbo University, Ningbo 315211, China

Funds: This project was supported by the National 863 Project (2012AA10A403), the Natural Science Foundation of Ningbo City of China (2014A610187) and the Scientific Research Foundation of Graduate School of Ningbo University (G14041)

More Information

Corresponding author: Jiong CHEN
Received Date: 2015-03-23
Rev Recd Date: 2015-04-24
Publish Date: 2015-05-08

Abstract

Abstract

Interleukin 1β (IL-1β), the first interleukin to be characterized, plays a key role in regulating the immune response. In this study, we determined the cDNA and genomic DNA sequences of the IL-1β gene from the large yellow croaker, Larimichthys crocea. Phylogenetic analysis indicated that the IL-1β (LcIL-1β) gene was most closely related to that of European seabass (Dicentrarchus labrax), sharing 67.8% amino acid identity. In healthy large yellow croaker, LcIL-1β transcription was detected in all tested tissues, with the highest level found in the head kidney. Upon Vibrio alginolyticus infection, LcIL-1β transcription in all tested tissues was significantly upregulated. Intraperitoneal injection of recombinant LcIL-1β (rLcIL-1β) improved the survival rate and reduced the tissue bacterial load after V. alginolyticus infection. In addition, rLcIL-1β induced monocytes/macrophages (MO/MΦ) chemotaxis and increased phagocytosis and bactericidal activity in vitro. These results suggest that LcIL-1β plays an important role in the large yellow croaker immune response against V. alginolyticus.
- Interleukin 1&beta,
- Large yellow croaker,
- Survival rate,
- Vibrio alginolyticus

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References(30)

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