1. Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China 2. Kunming Primate Research Center, the Chinese Academy of Sciences, Kunming 650223, China 3. The Research Center of Chinese National Human Genome at Shanghai, Shanghai 201203, China 4. Department of Chemistry and Life Science, Qujing Normal College, Qujing 655065, China 5. Graduate University of the Chinese Academy of Sciences, Beijing 100049, China
Total RNA was extracted from the prefrontal cortex (PFC) of rhesus monkeys with TRIzol reagent. mRNA was further purified from total RNA using a purifying kit for mRNA. According to the manual of cDNA Synthesis Kit (200401), ZAP-cDNA Synthesis Kit (200400)，and ZAP-cDNA GigapackⅢ Gold Cloning Kit (200450) from Stratagene, we constructed a cDNA-library of the PFC of rhesus monkeys. Our aim was to identify brain-function (such as cognition and memory) related genes and gene families. The cDNA library has a capacity of 2.0×10 6 clones, and 97.3% of them are recombinant clones. The average size of the inserted cDNA fragments is 1.0 kb with the majority of inserts larger than 0.5 kb, which meets the requirement of a standard cDNA library. The PFC cDNA library constructed will be useful in cloning and sequencing brain-function related genes, and in dissecting the alternative splicing patterns of genes expressed in the brain. Meanwhile, it will also serve as a useful resource in understanding the expression regulations within the brain.