Volume 26 Issue 3
May  2005
Turn off MathJax
Article Contents
HU Cheng-yu, XIE Zong-bo, ZHANG Yi-bing, CHEN Yu-dong, DENG Zheng-dong et al.. Binding of the Zα Domain from a Carassius auratus Protein Kinase PKR-like to Polyinosinic:Polycytidylic Acid. Zoological Research, 2005, 26(3): 237-242.
Citation: HU Cheng-yu, XIE Zong-bo, ZHANG Yi-bing, CHEN Yu-dong, DENG Zheng-dong et al.. Binding of the Zα Domain from a Carassius auratus Protein Kinase PKR-like to Polyinosinic:Polycytidylic Acid. Zoological Research, 2005, 26(3): 237-242.

Binding of the Zα Domain from a Carassius auratus Protein Kinase PKR-like to Polyinosinic:Polycytidylic Acid

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2005-06-22
  • Three fusion peptides,PZα1,PZα2 and PZα1Zα2 for Zα1 domain,Zα2 domain,and Zα1Zα2 domains of Carassius auratus PKR-like gene,respectively,were successfully expressed by a prokaryotic expression system and then purified by affinity chromatography. Gel mobility shift assay revealed that PZα1Zα2 rather than PZα1,PZα2,and mixture of PZα1 and PZα2,was capable to bind to polyinosinic∶polycytidylic acid (Poly I∶C) in vitro. In addition,all of the three fusion peptides all could form dimer,with strong dimerization for PZα2 and PZα1Zα2 but a relative weak one for PZα1. The results suggest that dsRNA, the by-product generated during virus replication in host cells,probably binds to the Zα domain of CaPKR-like and then regulates its physiological function.
  • loading
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (2393) PDF downloads(1703) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return