cDNA Cloning,mRNA Transcription of Sox9 Gene at Early Developmental Stages in Amur Sturgeons (Acipenser schrenckii)

CHEN Jin-ping; YUAN Hong-mei; WANG Bin; LIANG Bing; ZHANG Shu-yi

Volume 25 Issue 6

Nov. 2004

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Article Navigation > Zoological Research > 2004 > 25(6): 527-533

CHEN Jin-ping, YUAN Hong-mei, WANG Bin, LIANG Bing, ZHANG Shu-yi. cDNA Cloning,mRNA Transcription of Sox9 Gene at Early Developmental Stages in Amur Sturgeons (Acipenser schrenckii). Zoological Research, 2004, 25(6): 527-533.

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cDNA Cloning,mRNA Transcription of Sox9 Gene at Early Developmental Stages in Amur Sturgeons (Acipenser schrenckii)

1.
Technology Center of Sturgeon Breeding and Cultivation,Chinese Academy of Fishery Sciences,Beijing 100039,China 2.Zoology,the Chinese Academy of Sciences,Beijing 100080,China 3.Harbin Normal University,Harbin 150080,China

Received Date: 1900-01-01
Rev Recd Date: 1900-01-01
Publish Date: 2004-12-22

Abstract

Abstract

The Amur sturgeon (Acipenser schrenckii) Sox9 partial cDNA (1 140 bp) was cloned from cDNA of this species gonad by RACE-PCR,and the temporal and spatial expression patterns were analyzed by RT-PCR.RT-PCR showed that Sox9 mRNA is ubiquitously expressed at all the stages (1[+]-3[+]) checked of 8 tissues (heart,kidney,liver,eye,spleen,brain,testis and ovary).Sox9 expression levels were extremely low in the fry at 1 day after hatching,while mRNA expression level rose after 15 days.Expression pattern of gonad has not any difference between testis and ovary,which suggests that the function of Sox9 in the cartilage is conserved among vertebrates,while that in the gonad is quite different.
- Acipenser schrenckii,
- Sox9 gene,
- Expression pattern