Glaucoma model for stem cell transplantation research in New Zealand white rabbits

GUO Li-Yun; WEI Jing-Kuan; YANG Shang-Chuan; WANG Zheng-Bo

doi:10.3724/SP.J.1141.2012.02225

Volume 33 Issue 2

Mar. 2012

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GUO Li-Yun, WEI Jing-Kuan, YANG Shang-Chuan, WANG Zheng-Bo. Glaucoma model for stem cell transplantation research in New Zealand white rabbits. Zoological Research, 2012, (2): 225-230. doi: 10.3724/SP.J.1141.2012.02225

Citation:

GUO Li-Yun, WEI Jing-Kuan, YANG Shang-Chuan, WANG Zheng-Bo. Glaucoma model for stem cell transplantation research in New Zealand white rabbits. Zoological Research, 2012, (2): 225-230. doi: 10.3724/SP.J.1141.2012.02225

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Glaucoma model for stem cell transplantation research in New Zealand white rabbits

doi: 10.3724/SP.J.1141.2012.02225

1.
Department of Ophthalmology, the Fourth Affiliated Hospital of Kunming Medical College, Yunnan 650021, China;
2.
Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China;
3.
Graduate University of the Chinese Academy of Sciences, Beijing 100049, China

Received Date: 2011-12-14
Rev Recd Date: 2012-01-04
Publish Date: 2012-04-22

Abstract

Abstract

Glaucoma is a typical irreversible blind neurodegenerative disease for which there is no effective treatment for halting visual deterioration. The recent development of neural stem cells studies sheds light on a potential resolution for this disease. As a result, an appropriate glaucoma modeling method for stem cell transplantation study is needed. In the present study, Dexamethasone was injected unilaterally into the conjunctiva of New Zealand rabbit at the dose of 2.5 mg (5 mg/mL), three times a week. After eight weeks, the eye ground photography showed that the optic nerve head of the treated eye was expanded, and the blood vessel was geniculate compared to the control eye, while the ocular media remained transparent. The hematoxylin-eosin (HE) stain of the retinal nerve fiber layer (RNFL) sections showed optic neuron death in the treated eye. The Heidelberg Retina Tomography (HRT) results showed optic disk morphological changes consistent with the pathophysiology of glaucoma in the treated eye, including a decrease in the rim area (1.10?0.88) mm² and mean RNFL thickness (0.44?0.31) mm, and an increase in the cup/disk ratio 0.17?0.13.Then neural stem cells were injected into the vitreous body of the treated eye. After five months, surviving transplanted cells were observed. These results suggest a simple and reproducible chronic glaucoma model, which is appropriate for neural stem cell transplant research, has been successfully developed.
- Glaucoma,
- Animal model,
- Dexamethasone,
- Stem cells transplantation

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References(35)

References

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