Rhesus monkey embryonic stem cells differentiation, proliferation and allotransplantation

DONG Jin-Run; GUO Li-Yun; QU Jia-Gui; QI Ren-Li; WANG Wen-Chao; XIAO Chun-Jie; WANG Zheng-Bo

doi:10.3724/SP.J.1141.2012.01043

Volume 33 Issue 1

Jan. 2012

Turn off MathJax

Article Contents

Article Navigation > Zoological Research > 2012 > 33(1): 43-48

DONG Jin-Run, GUO Li-Yun, QU Jia-Gui, QI Ren-Li, WANG Wen-Chao, XIAO Chun-Jie, WANG Zheng-Bo. Rhesus monkey embryonic stem cells differentiation, proliferation and allotransplantation. Zoological Research, 2012, 33(1): 43-48. doi: 10.3724/SP.J.1141.2012.01043

Citation:

DONG Jin-Run, GUO Li-Yun, QU Jia-Gui, QI Ren-Li, WANG Wen-Chao, XIAO Chun-Jie, WANG Zheng-Bo. Rhesus monkey embryonic stem cells differentiation, proliferation and allotransplantation. Zoological Research, 2012, 33(1): 43-48. doi: 10.3724/SP.J.1141.2012.01043

Citation:

DONG Jin-Run, GUO Li-Yun, QU Jia-Gui, QI Ren-Li, WANG Wen-Chao, XIAO Chun-Jie, WANG Zheng-Bo. Rhesus monkey embryonic stem cells differentiation, proliferation and allotransplantation. Zoological Research, 2012, 33(1): 43-48. doi: 10.3724/SP.J.1141.2012.01043

PDF( 5093 KB)

Rhesus monkey embryonic stem cells differentiation, proliferation and allotransplantation

doi: 10.3724/SP.J.1141.2012.01043

1.
State Kay Laboratory of Brain and Cognitive Science, Institute of Biophysics, the Chinese Academy of Sciences, Beijing 100101, China;
2.
Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China;
3.
Life Sciences Faculty of Yunnan University, Kunming 650223, China;
4.
Department of Ophthalmology, the Fourth Affiliated Hospital of Kunming Medical College, Kunming 650021, China;
5.
School of Life Science, University of Science and Technology of China, Hefei 230026, China

Received Date: 2011-12-01
Rev Recd Date: 2011-12-22
Publish Date: 2012-02-22

Abstract

Abstract

To investigate the characteristics of rhesus monkey embryonic stem cells and to promote their clinical application, the differentiation and proliferation of rosettes neural stem cells from GFP marked rhesus monkey embryonic stem cells were studied The results showed that: 1) A stable and high-efficient neural differentiation system was established. More than 95% of the embryonic stem cells were differentiated into neural stem cells on the 12^th days of differentiation; 2) the rosettes neural stem cells differentiated from the rhesus monkey embryonic stem cells could maintain their rosettes-shape by proliferating with bFGF/EGF; 3) the neural stem cells could differentiate into neurons after transplanted into the rhesus monkey brain. In conclusion, the rosettes neural stem cells differentiated from rhesus monkey embryonic stem cells could maintain their characteristics after proliferation with bFGF/EGF and they could survive and differentiate into neurons after transplanted into the rhesus monkey brain, which strongly supports the clinical application of neural stem cells in the future.
- Rhesus monkey,
- Neural stem cells,
- Rosettes,
- Basic fibroblast growth factor,
- Epidermal growth factor

FullText(HTML)

References(64)

References

[1]	Amar AP, Zlokovic BV, Apuzzo ML. 2003. Endovascular restorative neurosurgery: a novel concept for molecular and cellular therapy of the nervous system
[J]. Neurosurgery, 52(2): 402-413.
[3]	Andressen C, Stöcker E, Klinz FJ, Lenka N, Hescheler J, Fleischmann B, Arnhold S, Addicks K. 2001. Nestin-specific green fluorescent protein expression in embryonic stem cell-derived neural precursor cells used for transplantation
[J]. Stem Cells, 19(5): 419-424.
[5]	Bain G, Kitchens D, Yao M, Huettner JE, Gottlieb DI. 1995. Embryonic stem cells express neuronal properties in vitro
[J]. Dev Biol, 168(2): 342-357.
[7]	Calhoun JD, Lambert NA, Mitalipova MM, Noggle SA, Lyons I, Condie BG, Stice SL. 2003. Differentiation of rhesus embryonic stem cells to neural progenitors and neurons
[J]. Biochem Biophys Res Commun, 306(1): 191-197.
[9]	Chen HW, Mao Y, Li B, Wei Q, Zhang J, Wang JH, Wang JH, Wang SF, Tan T, Zhang XZ, Li J, Ma YY, Ji WZ. 2008. Neural lineage development of rhesus monkey embryonic stem cells: Insight of neurogenesis and gliogenesis in vitro
[J]. Cell Res, 18(S1): s148.
[11]	Chen SS, Revoltella RP, Papini S, Michelini M, Fitzgerald W, Zimmerberg J, Margolis L. 2003. Multilineage differentiation of rhesus monkey embryonic stem cells in three-dimensional culture systems
[J]. Stem Cells, 21(3): 281-295.
[13]	Cohen S. 1962. Isolation of a mouse submaxillary gland protein accelerating incisor eruption and eyelid opening in the new-born animal
[J]. J Biol Chem, 237: 1555-1562.
[15]	Du ZW, Zhang SC. 2004. Neural differentiation from embryonic stem cells: which way?
[J]. Stem Cell Dev, 13(4): 372-381.
[17]	Elkabetz Y, Studer L. 2008. Human ESC-derived neural rosettes and neural stem cell progression
[J]. Cold Spring Harb Symp Quant Biol, 73: 377-387.
[19]	Gage FH. 2000. Mammalian neural stem cells
[J]. Science, 287(5457): 1433-1438.
[21]	Gospodarowicz D. 1975. Purification of a fibroblast growth factor from bovine pituitary
[J]. J Biol Chem, 250(7): 2515-2520.
[23]	Hong SH, Kang UJ, Isacson O, Kim KS. 2008. Neural precursors derived from human embryonic stem cells maintain long-term proliferation without losing the potential to differentiate into all three neural lineages, including dopaminergic neurons
[J]. J Neurochem, 104(2): 316-324.
[25]	Itsykson P, Ilouz N, Turetsky T, Goldstein RS, Pera MF, Fishbein I, Segal M, Reubinoff BE. 2005. Derivation of neural precursors from human embryonic stem cells in the presence of noggin
[J]. Mol Cell Neurosci, 30(1): 24-36.
[27]	Kanda S, Tamada Y, Yoshidome A, Hayashi I, Nishiyama T. 2004. Over-expression of bHLH genes facilitate neural formation of mouse embryonic stem (ES) cells in vitro
[J]. Int J Dev Neurosci, 22(3): 149-156.
[29]	Kim JH, Auerbach JM, Rodríguez-Gómez JA, Velasco I, Gavin D, Lumelsky N, Lee SH, Nguyen J, Sánchez-Pernaute R, Bankiewicz K, McKay R. 2002. Dopamine neurons derived from embryonic stem cells function in an animal model of Parkinson's disease
[J]. Nature, 418(6893): 50-56.
[31]	Koch P, Opitz T, Steinbeck JA, Ladewig J, Brüstle O. 2009. A rosette-type, self-renewing human ES cell-derived neural stem cell with potential for in vitro instruction and synaptic integration
[J]. Proc Natl Acad Sci USA, 106(9): 3225-3230.
[33]	Kuai XL, Gagliardi C, Flaat M, Bunnell BA. 2009. Differentiation of nonhuman primate embryonic stem cells along neural lineages
[J]. Differentiation, 77(3): 229-238.
[35]	Kuo HC, Pau KY, Yeoman RR, Mitalipov SM, Okano H, Wolf DP. 2003. Differentiation of monkey embryonic stem cells into neural lineages
[J]. Biol Reprod, 68(5): 1727-1735.
[37]	Li M, Pevny L, Lovell-Badge R, Smith A. 1998. Generation of purified neural precursors from embryonic stem cells by lineage selection
[J]. Curr Biol, 8(17): 971-974.
[39]	Li RR, Chen HW, Chen DL, Wang SF, Zhang J, Chen R, Ji WZ. 2008. Hepatocyte growth factor promotes the proliferation of the neural progenitors derived from rhesus monkey embryonic stem cells
[J]. Zool Res, 29(5): 518-528. (in Chinese)
[41]	Li T, Zheng J, Xie Y, Wang S, Zhang X, Li J, Jin L, Ma Y, Wolf DP, Zhou Q, Ji W. 2005. Transplantable neural progenitor populations derived from rhesus monkey embryonic stem cells
[J]. Stem Cells, 23(9): 1295-1303.
[43]	Modo M, Rezaie P, Heuschling P, Patel S, Male DK, Hodges H. 2003. Transplantation of neural stem cells in a rat model of stroke: assessment of short-term graft survival and acute host immunological response
[J]. Brain Res, 958(1): 70-82.
[45]	Muotri AR, Nakashima K, Toni N, Sandler VM, Gage FH. 2005. Development of functional human embryonic stem cell-derived neurons in mouse brain
[J]. Proc Natl Acad Sci USA, 102(51): 18644- 18648.
[47]	Murphy M, Drago J, Bartlett PF. 1990. Fibroblast growth factor stimulates the proliferation and differentiation of neural precursor cells in vitro
[J]. J Neurosci Res, 25(4): 463-475.
[49]	Okita K, Ichisaka T, Yamanaka S. 2007. Generation of germline-competent induced pluripotent stem cells
[J]. Nature, 448(7151): 313-317.
[51]	Pankratz MT, Li XJ, Lavaute TM, Lyons EA, Chen X, Zhang SC. 2007. Directed neural differentiation of human embryonic stem cells via an obligated primitive anterior stage
[J]. Stem Cells, 25(6): 1511-1520.
[53]	Reubinoff BE, Itsykson P, Turetsky T, Pera MF, Reinhartz E, Itzik A, Ben-Hur T. 2001. Neural progenitors from human embryonic stem cells
[J]. Nat Biotechnol, 19(12): 1134-1140.
[55]	Studer L, Tabar V, Mckay R. 1998. Transplantation of expanded mesencephalic precursors leads to recovery in parkinsonian rats
[J]. Nat Neurosci, 1(4): 290-295.
[57]	Takagi Y, Takahashi J, Saiki H, Morizane A, Hayashi T, Kishi Y, Fukuda H, Okamoto Y, Koyanagi M, Ideguchi M, Hayashi H, Imazato T, Kawasaki H, Suemori H, Omachi S, Iida H, Itoh N, Nakatsuji N, Sasai Y, Hashimoto N. 2005. Dopaminergic neurons generated from monkey embryonic stem cells function in a Parkinson primate model
[J]. J Clin Invest, 115(1): 102-109.
[59]	Wianny F, Bernat A, Huissoud C, Marcy G, Markossian S, Cortay V, Giroud P, Leviel V, Kennedy H, Savatier P, Dehay C. 2008. Derivation and cloning of a novel rhesus embryonic stem cell line stably expressing tau-green fluorescent protein
[J]. Stem Cells, 26(6): 1444-1453.
[61]	Wolf DP, Kuo HC, Pau KY, Lester L. 2004. Progress with nonhuman primate embryonic stem cells
[J]. Biol Reprod, 71(6): 1766-1771.
[63]	Yan YP, Yang DL, Zarnowska ED, Du ZW, Werbel B, Valliere C, Pearce RA, Thomson JA, Zhang SC. 2005. Directed differentiation of dopaminergic neuronal subtypes from human embryonic stem cells
[J]. Stem Cells, 23(6): 781-790.