Cloning of full-length coding sequence of tree shrew CD3&epsilon; and prediction of its molecular characteristics

LI Yi-Jiang; GAO Yue-Dong; GUO Yan; LU Cai-Xia; HUANG Jing-Fei; XIA Xue-Shan; DA

doi:10.3724/SP.J.1141.2010.05483

Volume 31 Issue 5

Sep. 2010

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Article Navigation > Zoological Research > 2010 > 31(5): 483-489

LI Yi-Jiang, GAO Yue-Dong, GUO Yan, LU Cai-Xia, HUANG Jing-Fei, XIA Xue-Shan, DA. Cloning of full-length coding sequence of tree shrew CD3ε and prediction of its molecular characteristics. Zoological Research, 2010, 31(5): 483-489. doi: 10.3724/SP.J.1141.2010.05483

Citation:

LI Yi-Jiang, GAO Yue-Dong, GUO Yan, LU Cai-Xia, HUANG Jing-Fei, XIA Xue-Shan, DA. Cloning of full-length coding sequence of tree shrew CD3ε and prediction of its molecular characteristics. Zoological Research, 2010, 31(5): 483-489. doi: 10.3724/SP.J.1141.2010.05483

Citation:

LI Yi-Jiang, GAO Yue-Dong, GUO Yan, LU Cai-Xia, HUANG Jing-Fei, XIA Xue-Shan, DA. Cloning of full-length coding sequence of tree shrew CD3ε and prediction of its molecular characteristics. Zoological Research, 2010, 31(5): 483-489. doi: 10.3724/SP.J.1141.2010.05483

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Cloning of full-length coding sequence of tree shrew CD3ε and prediction of its molecular characteristics

doi: 10.3724/SP.J.1141.2010.05483

1.
College of Animal Science and Veterinary Medicine, Yunnan Agricultural University, Kunming Yunnan 650201, China; 2. Military Medical Institute of Chengdu Military Garrison Center for Disease Control and Prevention, Kunming Yunnan 650032, China; 3. Immunobiology Laboratory, Key Laboratory of Animal Models and Human Disease Mechanisms, Kuming Institute of Zoology, the Chniese Academy of Science, Kunming Yunnan 650223, China; 4. Kunming Biological Diversity Regional Center of Large Apparatus and Equipment, the Chinese Academy of Sciences, Kunming Yunnan 650223, China; 5. State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming Yunnan 650223, China; 6. Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650224, China; 7. Institute of Medical Biology, Chinese Academy of Medical Science and Peking Union Medical College, Kunming 650118, China

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Author Bio:
LI Yi-Jiang,E-mail: liyijiang88.student@sina.com
Received Date: 2010-01-22
Rev Recd Date: 2010-09-15
Publish Date: 2010-10-22

Abstract

Abstract

The use of tree shrews (Tupaia belangeri) in human disease studies demands essential research tools, in particular cellular markers and their monoclonal antibodies for immunological studies. Here we cloned the full-length cDNAs encoding CD3ε from total RNA of the spleen, liver and peripheral blood of tree shrews and analyzed their structural characteristics in comparison with other mammals by Discovery Studio software. The results showed that the open reading frame sequence of tree shrew CD3ε was 582 bp, encoding 194 amino acids. The overall structure of tree shrew CD3ε protein was similar to its counterparts of other mammals, intracellular and transmembrane domain highly conserved. However, detailed analysis revealed two potential glycosylation sites and different surface charges in the extracellular domain. Availability of the entire open-reading-frame and related sequence information would therefore facilitate the preparation of monoclonal antibodies against tree shrew CD3 and further studies for its function.
- Tree shrews,
- CD3&epsilon,
- cDNA,
- Cloning,
- Structure,
- Function

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References(2)

References

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