Karyotypic evolution in family Hipposideridae (Chiroptera, Mammalia) revealed by comparative chromosome painting, G- and C-banding

MAO Xiu-Guang; WANG Jin-Huan; SU Wei-Ting; WANG Ying-Xiang

doi:10.3724/SP.J.1141.2010.05453

Volume 31 Issue 5

Sep. 2010

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Article Navigation > Zoological Research > 2010 > 31(5): 453-460

MAO Xiu-Guang, WANG Jin-Huan, SU Wei-Ting, WANG Ying-Xiang. Karyotypic evolution in family Hipposideridae (Chiroptera, Mammalia) revealed by comparative chromosome painting, G- and C-banding. Zoological Research, 2010, 31(5): 453-460. doi: 10.3724/SP.J.1141.2010.05453

Citation:

MAO Xiu-Guang, WANG Jin-Huan, SU Wei-Ting, WANG Ying-Xiang. Karyotypic evolution in family Hipposideridae (Chiroptera, Mammalia) revealed by comparative chromosome painting, G- and C-banding. Zoological Research, 2010, 31(5): 453-460. doi: 10.3724/SP.J.1141.2010.05453

Citation:

MAO Xiu-Guang, WANG Jin-Huan, SU Wei-Ting, WANG Ying-Xiang. Karyotypic evolution in family Hipposideridae (Chiroptera, Mammalia) revealed by comparative chromosome painting, G- and C-banding. Zoological Research, 2010, 31(5): 453-460. doi: 10.3724/SP.J.1141.2010.05453

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Karyotypic evolution in family Hipposideridae (Chiroptera, Mammalia) revealed by comparative chromosome painting, G- and C-banding

doi: 10.3724/SP.J.1141.2010.05453

1.
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, the Chinese Academy of Sciences，Kunming Yunnan 650223, China; 2. The Wellcome Trust Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, UK; 3. Graduate University of the Chinese Academy of Sciences, Beijing 100049, China

Funds: This study was partly supported by grants from the National Natural Science Foundation of China (30770293) and the Ministry of Science and Technology of China (2005DKA21502)

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Author Bio:
MAO Xiu-Guang
Received Date: 2010-01-26
Rev Recd Date: 2010-07-07
Publish Date: 2010-10-22

Abstract

Abstract

Comparing to its sister-family (Rhinolophidae), Hipposideridae was less studied by cytogenetic approaches. Only a few high-resolution G-banded karyotypes have been reported so far, and most of the conclusions on the karyotypic evolution in Hipposideridae were based on conventional Giemsa-staining. In this study, we applied comparative chromosome painting, a method of choice for genome-wide comparison at the molecular level, and G- and C-banding to establish comparative map between five hipposiderid species from China, using a whole set of chromosome-specific painting probes from one of them (Aselliscus stoliczkanus). G-band and C-band comparisons between homologous segments defined by chromosome painting revealed that Robertsonian translocations, paracentric inversions and heterochromatin addition could be the main mechanism of chromosome evolution in Hipposideridae. Comparative analysis of the conserved chromosomal segments among five hipposiderid species and outgroup species suggests that bi-armed chromosomes should be included into the ancestral karyotype of Hipposideridae, which was previously believed to be exclusively composed of acrocentric chromosomes.
- Comparative chromosome painting,
- Robertsonian translocation,
- Paracentric inversion,
- Hipposideridae,
- Chiroptera

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References

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