Volume 30 Issue 6
Nov.  2009
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CHEN Ge-fei, ZHANG Yun-long, LIN Sen-zhu, LI Wen, MENG Qing. Extraction and Electroelution of Spiders’HMW-gDNA. Zoological Research, 2009, 30(6): 627-632. doi: 10.3724/SP.J.1141.2009.06627
Citation: CHEN Ge-fei, ZHANG Yun-long, LIN Sen-zhu, LI Wen, MENG Qing. Extraction and Electroelution of Spiders’HMW-gDNA. Zoological Research, 2009, 30(6): 627-632. doi: 10.3724/SP.J.1141.2009.06627

Extraction and Electroelution of Spiders’HMW-gDNA

doi: 10.3724/SP.J.1141.2009.06627
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  • Corresponding author: MENG Qing
  • Received Date: 2009-05-07
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2009-12-22
  • Extraction of high molecular weight genomic DNA (HMW-gDNA) from spider organs and construction of an electroelution system for obtaining HMW-gDNA efficiently are described. We improved the CTAB method to isolate genomic DNA from spider pectoral muscle tissues, constructed a novel electroelution system with the use of 2-mL centrifuge tube and dialysis membrane, and utilized this self-constructed electroelution system to recover target HMW-gDNA from agarose gels. The HMW-gDNA extracted using the improved CTAB method could be over 48.5 kb in size. By trapping the HMW-gDNA with dialysis membrane, the electroelution system could recover target HMW-gDNA from agarose gels rapidly and without affecting the integrity of HMW-gDNA. The recovery rate was over 75% with the OD260/OD280 ranging from 1.8 to 2.0. The results demonstrate that the improved CTAB method can be used to extract spider HMW-gDNA, and the electroelution system is a general method for recovering target HMW-gDNA from agarose gels with low cost, simplicity, high efficiency and good practicability.
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