CHANG Yu-mei, XU Wan-tu, CHI Bing-jie, GAO Guo-qiang, HAN Qi-xia, HE Wei, SUN Xiao-we. Kinship Analysis of One Broodstock Population of Large Yellow Croaker Pseudosciaena crocea. Zoological Research, 2009, 30(6): 620-626. doi: 10.3724/SP.J.1141.2009.06620
Citation:
CHANG Yu-mei, XU Wan-tu, CHI Bing-jie, GAO Guo-qiang, HAN Qi-xia, HE Wei, SUN Xiao-we. Kinship Analysis of One Broodstock Population of Large Yellow Croaker Pseudosciaena crocea. Zoological Research, 2009, 30(6): 620-626. doi: 10.3724/SP.J.1141.2009.06620
CHANG Yu-mei, XU Wan-tu, CHI Bing-jie, GAO Guo-qiang, HAN Qi-xia, HE Wei, SUN Xiao-we. Kinship Analysis of One Broodstock Population of Large Yellow Croaker Pseudosciaena crocea. Zoological Research, 2009, 30(6): 620-626. doi: 10.3724/SP.J.1141.2009.06620
Citation:
CHANG Yu-mei, XU Wan-tu, CHI Bing-jie, GAO Guo-qiang, HAN Qi-xia, HE Wei, SUN Xiao-we. Kinship Analysis of One Broodstock Population of Large Yellow Croaker Pseudosciaena crocea. Zoological Research, 2009, 30(6): 620-626. doi: 10.3724/SP.J.1141.2009.06620
Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Harbin 150070, China; 2. Xiangshan Seaport Aquatic Seedling&fingerling Limited Company, Xiangshan 315702, China;3. College of Fishery and Life Science, Shanghai Ocean University, Shanghai 201306, China;4. Life Science of Technology Institute, Dalian Fisheries University, Dalian 116023, China
Present broodstocks of large yellow croaker are borne from extremely small numbers of base population. Thus, it is necessary to analyze kinship of broodstocks in order to avoid inbreeding that will bring out the reduction of individual survival and growth. This paper reports kinship reconstruction and genetic diversity in 103 broodstocks of large yellow croaker by utilizing 23 microsatellite markers. Genetic diversities of 103 croakers at 23 loci pronounce that there are 134 alleles in total and an average of 5.82, and the observed average heterozygosity of 0.599 3, demonstrating that these broodstocks still maintain genetic variability to some extent. The results of sibling groups reconstructed are not identical using two methods of liklihood and 2-allele recombinatorial optimization. However, the evidence of close relationship between broodstocks is confirmed. The mating combinations are compared between these two methods, as a result of 85% identity and a final selection of 2-allele method. This study aims at finding a better way to avoid inbreeding occurred in broodstocks, and facilitating the aquaculture market of large yellow croaker, meanwhile, offering methods and statistical models to artificial propagation in other marine fish species.