GUO Ji-tong, LI Xue-feng, Shahnaz Fida, GOU Kemian, Nakisa Malakooti, ZHANG Chun-fan. The Potential of Rat Inner Cell Mass and Fetal Neural Stem Cells to Generate Chimeras. Zoological Research, 2009, 30(2): 158-164. doi: 10.3724/SP.J.1141.2009.02158
Citation:
GUO Ji-tong, LI Xue-feng, Shahnaz Fida, GOU Kemian, Nakisa Malakooti, ZHANG Chun-fan. The Potential of Rat Inner Cell Mass and Fetal Neural Stem Cells to Generate Chimeras. Zoological Research, 2009, 30(2): 158-164. doi: 10.3724/SP.J.1141.2009.02158
GUO Ji-tong, LI Xue-feng, Shahnaz Fida, GOU Kemian, Nakisa Malakooti, ZHANG Chun-fan. The Potential of Rat Inner Cell Mass and Fetal Neural Stem Cells to Generate Chimeras. Zoological Research, 2009, 30(2): 158-164. doi: 10.3724/SP.J.1141.2009.02158
Citation:
GUO Ji-tong, LI Xue-feng, Shahnaz Fida, GOU Kemian, Nakisa Malakooti, ZHANG Chun-fan. The Potential of Rat Inner Cell Mass and Fetal Neural Stem Cells to Generate Chimeras. Zoological Research, 2009, 30(2): 158-164. doi: 10.3724/SP.J.1141.2009.02158
CopyRat Pty Ltd, 2731 Wright Street, Clayton, Victoria 3168, Australia; 2. Monash Immunology and Stem Cell Laboratories (MISCL), Monash Science Technology Research and Innovation Precinct (STRIP), Building 75, Monash University, Wellington Road, Clayton, Victoria 3800, Australia; 3. School of Life Science, South China Normal University, Guangzhou Guangdong 510631, P. R. China; 4. Monash Animal Service Building 41, Monash University, Wellington Road, Clayton, Victoria 3800, Australia
The rat chimera is an important animal model for the study of complex human diseases. In the present study we evaluated the chimeric potential of rat inner cell masses (ICMs) and fetal neural stem (FNS) cells. In result, three rat chimeras were produced by day 5 (D5) Sprague-Dawley (SD) blastocysts injected with ICMs derived from day 6 (D6) and D5 Dark Agouti (DA) blastocysts; four rat chimeras had been generated by D5 DA blastocyst injected with D5 SD ICMs. For the requirement of gene modification, cultured rat inner cell mass cells were assessed to produce chimeras, but no chimeras were generated from injected embryos. The potential to generate chimeras from rFNS and transfected rFNS cells were tested, but no chimeric pups were produced. Only 2 of 41 fetuses derived from D5 DA blastocyst injection with SD LacZ transfected rFNS cells showed very low number of LacZ positive cells in the section. These results indicate that DA and SD rat ICMs are able to contribute to chimeras, but their potential decreases significantly after culture in vitro (P<0.05), and rFNS cells only have the potential to contribute to early fetal development.