Volume 29 Issue 4
Jul.  2008
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LU Xiao-peng, LI Jian, SUN Zheng-hua, ZHAO Na, LIU Jie, ZHANG Hua-tang. A “2+2 days” Fast Protocol for the Generation of Dendritic Cells from Human Blood Monocytes . Zoological Research, 2008, 29(4): 415-420. doi: 10.3724/SP.J.1141.2008.04415
Citation: LU Xiao-peng, LI Jian, SUN Zheng-hua, ZHAO Na, LIU Jie, ZHANG Hua-tang. A “2+2 days” Fast Protocol for the Generation of Dendritic
Cells from Human Blood Monocytes
. Zoological Research, 2008, 29(4): 415-420. doi: 10.3724/SP.J.1141.2008.04415

A “2+2 days” Fast Protocol for the Generation of Dendritic
Cells from Human Blood Monocytes

doi: 10.3724/SP.J.1141.2008.04415
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  • Corresponding author: ZHANG Hua-tang
  • Received Date: 2008-04-03
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2008-08-22
  • A “2+2 days” fast protocol for the generation of dendritic cells(DC) from high purity human monocytes in vitro has been established. During the 2-step differentiation and activation process, we demonstrated that 2 days of culture with GM-CSF and IL-4 were sufficient to generate immature DCs capable of antigen uptake. Similarly the mature DCs were activated with a cocktail of rTNF-α, IL-1β, IL-6, and PGE2 from immature DC in 2 days. This “2+2” fast DC had the same phenotype and function as the “6+2” standard DC.
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