Zhiqin Ren, Ding Ye, Naike Su, Chaofan Wang, Lijia He, Houpeng Wang, Mudan He, Yonghua Sun. 2024. foxl2l is a germ cell-intrinsic gatekeeper of oogenesis in zebrafish. Zoological Research, 45(5): 1116-1130. DOI: 10.24272/j.issn.2095-8137.2024.046
Citation: Zhiqin Ren, Ding Ye, Naike Su, Chaofan Wang, Lijia He, Houpeng Wang, Mudan He, Yonghua Sun. 2024. foxl2l is a germ cell-intrinsic gatekeeper of oogenesis in zebrafish. Zoological Research, 45(5): 1116-1130. DOI: 10.24272/j.issn.2095-8137.2024.046

foxl2l is a germ cell-intrinsic gatekeeper of oogenesis in zebrafish

  • Zebrafish serve as a valuable model organism for studying germ cell biology and reproductive processes. The AB strain of zebrafish is proposed to exhibit a polygenic sex determination system, where most males initially develop juvenile ovaries before committing to male fate. In species with chromosomal sex determination, gonadal somatic cells are recognized as key determinants of germ cell fate. Notably, the loss of germ cells in zebrafish leads to masculinization, implying that germ cells harbor an intrinsic feminization signal. However, the specific signal triggering oogenesis in zebrafish remains unclear. In the present study, we identifiedfoxl2las an oocyte progenitor-specific gene essential for initiating oogenesis in germ cells. Results showed thatfoxl2l-knockout zebrafish bypassed the juvenile ovary stage and exclusively developed into fertile males. Further analysis revealed that loss offoxl2lhindered the initiation of oocyte-specific meiosis and prevented entry into oogenesis, leading to premature spermatogenesis during early gonadal development. Furthermore, while mutation of the pro-male genedmrt1led to fertile female differentiation, simultaneous disruption offoxl2lindmrt1mutants completely blocked oogenesis, with a large proportion of germ cells arrested as germline stem cells, highlighting the crucial role offoxl2lin oogenesis. Overall, this study highlights the unique function offoxl2las a germ cell-intrinsic gatekeeper of oogenesis in zebrafish.
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