Hypoxia tolerance in fish depends on catabolic preference between lipids and carbohydrates

Qiang Ma; Yuan Luo; Jia Zhong; Samwel Mchele Limbu; Ling-Yu Li; Li-Qiao Chen; Fang Qiao; Mei-Ling Zhang; Qiang Lin; Zhen-Yu Du

doi:10.24272/j.issn.2095-8137.2023.098

Volume 44 Issue 5

Sep. 2023

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Article Navigation > Zoological Research > 2023 > 44(5): 954-966

Qiang Ma, Yuan Luo, Jia Zhong, Samwel Mchele Limbu, Ling-Yu Li, Li-Qiao Chen, Fang Qiao, Mei-Ling Zhang, Qiang Lin, Zhen-Yu Du. Hypoxia tolerance in fish depends on catabolic preference between lipids and carbohydrates. Zoological Research, 2023, 44(5): 954-966. doi: 10.24272/j.issn.2095-8137.2023.098

Citation:

Qiang Ma, Yuan Luo, Jia Zhong, Samwel Mchele Limbu, Ling-Yu Li, Li-Qiao Chen, Fang Qiao, Mei-Ling Zhang, Qiang Lin, Zhen-Yu Du. Hypoxia tolerance in fish depends on catabolic preference between lipids and carbohydrates. Zoological Research, 2023, 44(5): 954-966. doi: 10.24272/j.issn.2095-8137.2023.098

Citation:

Qiang Ma, Yuan Luo, Jia Zhong, Samwel Mchele Limbu, Ling-Yu Li, Li-Qiao Chen, Fang Qiao, Mei-Ling Zhang, Qiang Lin, Zhen-Yu Du. Hypoxia tolerance in fish depends on catabolic preference between lipids and carbohydrates. Zoological Research, 2023, 44(5): 954-966. doi: 10.24272/j.issn.2095-8137.2023.098

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Hypoxia tolerance in fish depends on catabolic preference between lipids and carbohydrates

doi: 10.24272/j.issn.2095-8137.2023.098

1.
LANEH, School of Life Sciences, East China Normal University, Shanghai 200241, China
2.
Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, Shandong 266071, China
3.
CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou, Guangdong 510301, China
4.
School of Aquatic Sciences and Fisheries Technology, University of Dar es Salaam, Dar es Salaam 60091, Tanzania

All sequencing data in the study were uploaded to the NCBI database under accession number SUB12161610/PRJNA891768, National Genomics Data Center under GSA accession number CRA011961 (https://bigd.big.ac.cn/gsa/browse/CRA011961), and Science Data Bank under the link https://doi.org/10.57760/sciencedb.09691.
Supplementary data to this article can be found online.
The authors declare that they have no competing interests.
Q.M. and Z.Y.D. designed and conducted the research. Q.M., Y.L., J.Z., and L.Y.L performed the experiments and analyzed the data. L.Q.C., F.Q., M.L.Z., and Q.L. provided essential materials and technical support. Q.M., Z.Y.D., and S.M.L wrote and revised the manuscript. All authors read and approved the final version of the manuscript.

Funds: This work was supported by the National Natural Science Foundation of China (31830102, 32202950)

More Information

Corresponding author: E-mail: zydu@bio.ecnu.edu.cn
Received Date: 2023-05-15
Accepted Date: 2023-05-19

Published Online: 2023-09-12

Publish Date: 2023-09-18

Abstract

Abstract

Hypoxia is a common environmental stress factor in aquatic organisms, which varies among fish species. However, the mechanisms underlying the ability of fish species to tolerate hypoxia are not well known. Here, we showed that hypoxia response in different fish species was affected by lipid catabolism and preference for lipid or carbohydrate energy sources. Activation of biochemical lipid catabolism through peroxisome proliferator-activated receptor alpha (Pparα) or increasing mitochondrial fat oxidation in tilapia decreased tolerance to acute hypoxia by increasing oxygen consumption and oxidative damage and reducing carbohydrate catabolism as an energy source. Conversely, lipid catabolism inhibition by suppressing entry of lipids into mitochondria in tilapia or individually knocking out three key genes of lipid catabolism in zebrafish increased tolerance to acute hypoxia by decreasing oxygen consumption and oxidative damage and promoting carbohydrate catabolism. However, anaerobic glycolysis suppression eliminated lipid catabolism inhibition-promoted hypoxia tolerance in adipose triglyceride lipase (atgl) mutant zebrafish. Using 14 fish species with different trophic levels and taxonomic status, the fish preferentially using lipids for energy were more intolerant to acute hypoxia than those preferentially using carbohydrates. Our study shows that hypoxia tolerance in fish depends on catabolic preference for lipids or carbohydrates, which can be modified by regulating lipid catabolism.
- Hypoxia tolerance,
- Catabolic preference,
- Lipid,
- Carbohydrate,
- Oxidative damage

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References(59)

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