LncRNA pol-lnc78 as a ceRNA regulates antibacterial responses via suppression of pol-miR-n199-3p-mediated SARM down-regulation in <i>Paralichthys</i> <i>olivaceus</i>

Xian-Hui Ning; Bing Han; Ye Peng; Shao-Wu Yin

doi:10.24272/j.issn.2095-8137.2022.520

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Xian-Hui Ning, Bing Han, Ye Peng, Shao-Wu Yin. LncRNA pol-lnc78 as a ceRNA regulates antibacterial responses via suppression of pol-miR-n199-3p-mediated SARM down-regulation in Paralichthys olivaceus. Zoological Research, 2024, 45(1): 25-35. doi: 10.24272/j.issn.2095-8137.2022.520

Citation:

Xian-Hui Ning, Bing Han, Ye Peng, Shao-Wu Yin. LncRNA pol-lnc78 as a ceRNA regulates antibacterial responses via suppression of pol-miR-n199-3p-mediated SARM down-regulation in Paralichthys olivaceus. Zoological Research, 2024, 45(1): 25-35. doi: 10.24272/j.issn.2095-8137.2022.520

Citation:

Xian-Hui Ning, Bing Han, Ye Peng, Shao-Wu Yin. LncRNA pol-lnc78 as a ceRNA regulates antibacterial responses via suppression of pol-miR-n199-3p-mediated SARM down-regulation in Paralichthys olivaceus. Zoological Research, 2024, 45(1): 25-35. doi: 10.24272/j.issn.2095-8137.2022.520

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LncRNA pol-lnc78 as a ceRNA regulates antibacterial responses via suppression of pol-miR-n199-3p-mediated SARM down-regulation in Paralichthys olivaceus

doi: 10.24272/j.issn.2095-8137.2022.520

Xian-Hui Ning^{1, 2, 3},
Bing Han¹,
Ye Peng¹,
Shao-Wu Yin^{1, 3
,
,}

1.
College of Marine Science and Engineering, Nanjing Normal University, Nanjing, Jiangsu 210023, China
2.
CAS Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, Shandong 266071, China
3.
Co-Innovation Center for Marine Bio-Industry Technology of Jiangsu Province, Lianyungang, Jiangsu 222005, China

Supplementary data to this article can be found online.
The authors declare that they have no competing interests.
Conceptualization, X.H.N. and S.W.Y.; Funding acquisition, X.H.N. and S.W.Y.; Methodology, X.H.N.; Investigation, B.H., Y.P., and X.H.N.; Resources, X.H.N.; Original draft, X.H.N.; Review & editing, X.H.N. and S.W.Y. All authors read and approved the final version of the manuscript.

Funds: This work was supported by the National Natural Science Foundation of China (42006082), Natural Science Foundation of Jiangsu Province of China (BK20221323), “JBGS” Project of Seed Industry Revitalization in Jiangsu Province (JBGS [2021] 034), and State Key Laboratory of Developmental Biology of Freshwater Fish (2021KF009)

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Corresponding author: E-mail: yinshaowu@163.com
Received Date: 2023-04-27
Accepted Date: 2023-05-17

Published Online: 2023-09-08

Abstract

Abstract

Long non-coding RNAs (lncRNAs) function as key modulators in mammalian immunity, particularly due to their involvement in lncRNA-mediated competitive endogenous RNA (ceRNA) crosstalk. Despite their recognized significance in mammals, research on lncRNAs in lower vertebrates remains limited. In the present study, we characterized the first immune-related lncRNA (pol-lnc78) in the teleost Japanese flounder (Paralichthys olivaceus). Results indicated that pol-lnc78 acted as a ceRNA for pol-miR-n199-3p to target the sterile alpha and armadillo motif-containing protein (SARM), the fifth discovered member of the Toll/interleukin 1 (IL-1) receptor (TIR) adaptor family. This ceRNA network regulated the antibacterial responses of flounder via the Toll-like receptor (TLR) signaling pathway. Specifically, SARM acted as a negative regulator and exacerbated bacterial infection by inhibiting the expression of inflammatory cytokines IL-1β and tumor necrosis factor-α (TNF-α). Pol-miR-n199-3p reduced SARM expression by specifically interacting with the 3’ untranslated region (UTR), thereby promoting SARM-dependent inflammatory cytokine expression and protecting the host against bacterial dissemination. Furthermore, pol-lnc78 sponged pol-miR-n199-3p to ameliorate the inhibition of SARM expression. During infection, the negative regulators pol-lnc78 and SARM were significantly down-regulated, while pol-miR-n199-3p was significantly up-regulated, thus favoring host antibacterial defense. These findings provide novel insights into the mechanisms underlying fish immunity and open new horizons to better understand ceRNA crosstalk in lower vertebrates.
- LncRNA,
- SARM,
- miRNA,
- ceRNA,
- Antibacterial response

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References(69)

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