<i>Dusp1</i> regulates thermal tolerance limits in zebrafish by maintaining mitochondrial integrity

Ying Wang; Hua-Min Wang; Yan Zhou; Ling-Hong Hu; Jing-Ming Wan; Ji-Hui Yang; Hong-Bo Niu; Xiu-Ping Hong; Peng Hu; Liang-Biao Chen

doi:10.24272/j.issn.2095-8137.2022.397

Volume 44 Issue 1

Jan. 2023

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Article Navigation > Zoological Research > 2023 > 44(1): 126-141

Ying Wang, Hua-Min Wang, Yan Zhou, Ling-Hong Hu, Jing-Ming Wan, Ji-Hui Yang, Hong-Bo Niu, Xiu-Ping Hong, Peng Hu, Liang-Biao Chen. Dusp1 regulates thermal tolerance limits in zebrafish by maintaining mitochondrial integrity. Zoological Research, 2023, 44(1): 126-141. doi: 10.24272/j.issn.2095-8137.2022.397

Citation:

Ying Wang, Hua-Min Wang, Yan Zhou, Ling-Hong Hu, Jing-Ming Wan, Ji-Hui Yang, Hong-Bo Niu, Xiu-Ping Hong, Peng Hu, Liang-Biao Chen. Dusp1 regulates thermal tolerance limits in zebrafish by maintaining mitochondrial integrity. Zoological Research, 2023, 44(1): 126-141. doi: 10.24272/j.issn.2095-8137.2022.397

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Dusp1 regulates thermal tolerance limits in zebrafish by maintaining mitochondrial integrity

doi: 10.24272/j.issn.2095-8137.2022.397

Ying Wang^{1, 2, 3},
Hua-Min Wang^{1, 2, 3},
Yan Zhou^{1, 2, 3},
Ling-Hong Hu^{1, 2, 3},
Jing-Ming Wan^{1, 2, 3},
Ji-Hui Yang^{1, 2, 3},
Hong-Bo Niu^{1, 2, 3},
Xiu-Ping Hong^{1, 2, 3},
Peng Hu^{1, 2, 3},
Liang-Biao Chen^{1, 2, 3
,
,}

1.
International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai 200120, China
2.
Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai 200120, China
3.
Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 200120, China

All Illumina RNA sequencing data from this project were deposited at NCBI under BioProjectID PRJNA602594, National Genomics Data Center (GSA: CRA008815), and Science Data Bank (DOI: 10.57760/sciencedb.06296).
Supplementary data to this article can be found online.
The authors declare that they have no competing interests.
Y.W. and L.B.C. designed the experiment, completed data analysis, and wrote the manuscript. H.M.W., L.H.H., J.M.W., J.H.Y., H.B.N., and X.P.H. performed the experiments. Y.Z. and P.H. helped with analyses and constructive discussion. All authors read and approved the final version of the manuscript.

Funds: This work was supported by the National Key Research and Development Program of China (2018YFD0900601) and National Natural Science Foundation of China (32130109)

More Information

Corresponding author: E-mail: lbchen@shou.edu.cn
Received Date: 2022-10-26
Accepted Date: 2022-11-23

Published Online: 2022-11-23

Publish Date: 2023-01-18

Abstract

Abstract

Temperature tolerance restricts the distribution of a species. However, the molecular and cellular mechanisms that set the thermal tolerance limits of an organism are poorly understood. Here, we report on the function of dual-specificity phosphatase 1 (DUSP1) in thermal tolerance regulation. Notably, we found that dusp1^-/- zebrafish grew normally but survived within a narrowed temperature range. The higher susceptibility of these mutant fish to both cold and heat challenges was attributed to accelerated cell death caused by aggravated mitochondrial dysfunction and over-production of reactive oxygen species in the gills. The DUSP1-MAPK-DRP1 axis was identified as a key pathway regulating these processes in both fish and human cells. These observations suggest that DUSP1 may play a role in maintaining mitochondrial integrity and redox homeostasis. We therefore propose that maintenance of cellular redox homeostasis may be a key mechanism for coping with cellular thermal stress and that the interplay between signaling pathways regulating redox homeostasis in the most thermosensitive tissue (i.e., gills) may play an important role in setting the thermal tolerance limit of zebrafish.
- Redox homeostasis,
- Zebrafish,
- Ectothermal,
- Mitochondrial fission,
- Thermal stress,
- MAPK

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References(92)

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