Optimization of sgRNA expression strategy to generate multiplex gene-edited pigs

Ju-Qing Zhang; Jian-Xiong Guo; Xiao-Jie Wu; Yu Wang; Wei Yue; Zhuo-Hui Li; Wei Wang; Xin-Fang Wei; Xiao-Long Wu; Qiao-Yan Shen; An-Min Lei; Hong-Jiang Wei; Jin-Lian Hua

doi:10.24272/j.issn.2095-8137.2022.244

Volume 43 Issue 6

Nov. 2022

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Article Navigation > Zoological Research > 2022 > 43(6): 1005-1008

Ju-Qing Zhang, Jian-Xiong Guo, Xiao-Jie Wu, Yu Wang, Wei Yue, Zhuo-Hui Li, Wei Wang, Xin-Fang Wei, Xiao-Long Wu, Qiao-Yan Shen, An-Min Lei, Hong-Jiang Wei, Jin-Lian Hua. Optimization of sgRNA expression strategy to generate multiplex gene-edited pigs. Zoological Research, 2022, 43(6): 1005-1008. doi: 10.24272/j.issn.2095-8137.2022.244

Citation:

Ju-Qing Zhang, Jian-Xiong Guo, Xiao-Jie Wu, Yu Wang, Wei Yue, Zhuo-Hui Li, Wei Wang, Xin-Fang Wei, Xiao-Long Wu, Qiao-Yan Shen, An-Min Lei, Hong-Jiang Wei, Jin-Lian Hua. Optimization of sgRNA expression strategy to generate multiplex gene-edited pigs. Zoological Research, 2022, 43(6): 1005-1008. doi: 10.24272/j.issn.2095-8137.2022.244

Citation:

Ju-Qing Zhang, Jian-Xiong Guo, Xiao-Jie Wu, Yu Wang, Wei Yue, Zhuo-Hui Li, Wei Wang, Xin-Fang Wei, Xiao-Long Wu, Qiao-Yan Shen, An-Min Lei, Hong-Jiang Wei, Jin-Lian Hua. Optimization of sgRNA expression strategy to generate multiplex gene-edited pigs. Zoological Research, 2022, 43(6): 1005-1008. doi: 10.24272/j.issn.2095-8137.2022.244

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Optimization of sgRNA expression strategy to generate multiplex gene-edited pigs

doi: 10.24272/j.issn.2095-8137.2022.244

1.
College of Veterinary Medicine, Shaanxi Centre of Stem Cells Engineering & Technology, Northwest A&F University, Yangling, Shaanxi 712100, China
2.
State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, Yunnan 650201, China
3.
College of Animal Sciences & Technology, Northwest A&F University, Yangling, Shaanxi 712100 China
4.
National Institute of Biological Sciences, Beijing 102206, China

Supplementary data to this article can be found online.
The authors declare that they have no competing interests.
J.L.H., H.J.W., and J.Q.Z. designed the study and wrote the manuscript. J.L.H., H.J.W., J.Q.Z., Y.W., Z.H.L., W.W., J.X.G., X.J.W., W.Y., X.L.W., Q.Y.S., A.M.L., and X.F.W. performed the experiments and analyzed the data. All authors read and approved the final version of the manuscript.
#Authors contributed equally to this work

Funds: This work was supported by the National Natural Science Foundation of China (32072806), Shaanxi Province Science and Technology Innovation Team (2019TD-036), and Key Program of Shaanxi Province Science and Technology (2022NY-044)

More Information

Corresponding author: E-mail: hongjiangwei@126.com; jinlianhua@nwsuaf.edu.cn
Received Date: 2022-08-14
Accepted Date: 2022-10-10

Published Online: 2022-10-09

Publish Date: 2022-11-18

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References(12)

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