Volume 42 Issue 5
Sep.  2021
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Zu-Bing Cao, Di Gao, Hui-Qun Yin, Hui Li, Teng-Teng Xu, Meng-Ya Zhang, Xin Wang, Qiu-Chen Liu, Ye-Lian Yan, Yang-Yang Ma, Tong Yu, Yun-Sheng Li, Yun-Hai Zhang. Chromatin remodeler INO80 mediates trophectoderm permeability barrier to modulate morula-to-blastocyst transition. Zoological Research, 2021, 42(5): 562-573. doi: 10.24272/j.issn.2095-8137.2021.075
Citation: Zu-Bing Cao, Di Gao, Hui-Qun Yin, Hui Li, Teng-Teng Xu, Meng-Ya Zhang, Xin Wang, Qiu-Chen Liu, Ye-Lian Yan, Yang-Yang Ma, Tong Yu, Yun-Sheng Li, Yun-Hai Zhang. Chromatin remodeler INO80 mediates trophectoderm permeability barrier to modulate morula-to-blastocyst transition. Zoological Research, 2021, 42(5): 562-573. doi: 10.24272/j.issn.2095-8137.2021.075

Chromatin remodeler INO80 mediates trophectoderm permeability barrier to modulate morula-to-blastocyst transition

doi: 10.24272/j.issn.2095-8137.2021.075
#Authors contributed equally to this work
Funds:  This work was supported by the Anhui Provincial Natural Science Foundation (1908085MC97, 2008085MC85), National Natural Science Foundation of China (31802059, 31902226), Hefei Innovation and Entrepreneurship Support Plan for Returnee Scholar (03082009), and Anhui Provincial Innovation and Entrepreneurship Support Plan for Returnee Scholar (2020LCX015)
More Information
  • Corresponding author: E-mail: yunhaizhang@ahau.edu.cn
  • Received Date: 2021-06-15
  • Accepted Date: 2021-07-30
  • Available Online: 2021-08-02
  • Publish Date: 2021-09-18
  • Inositol requiring mutant 80 (INO80) is a chromatin remodeler that regulates pluripotency maintenance of embryonic stem cells and reprogramming of somatic cells into pluripotent stem cells. However, the roles and mechanisms of INO80 in porcine pre-implantation embryonic development remain largely unknown. Here, we show that INO80 modulates trophectoderm epithelium permeability to promote porcine blastocyst development. The INO80 protein is highly expressed in the nuclei during morula-to-blastocyst transition. Functional studies revealed that RNA interference (RNAi)-mediated knockdown of INO80 severely blocks blastocyst formation and disrupts lineage allocation between the inner cell mass and trophectoderm. Mechanistically, single-embryo RNA sequencing revealed that INO80 regulates multiple genes, which are important for lineage specification, tight junction assembly, and fluid accumulation. Consistent with the altered expression of key genes required for tight junction assembly, a permeability assay showed that paracellular sealing is defective in the trophectoderm epithelium of INO80 knockdown blastocysts. Importantly, aggregation of 8-cell embryos from the control and INO80 knockdown groups restores blastocyst development and lineage allocation via direct complementation of the defective trophectoderm epithelium. Taken together, these results demonstrate that INO80 promotes blastocyst development by regulating the expression of key genes required for lineage specification, tight junction assembly, and fluid accumulation.
  • #Authors contributed equally to this work
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