Volume 42 Issue 3
May  2021
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Cui Yang, Zhi-Lan Liu, Jun Wang, Xian-Le Bu, Yan-Jiang Wang, Yang Xiang. Parabiosis modeling: protocol, application and perspectives. Zoological Research, 2021, 42(3): 253-261. doi: 10.24272/j.issn.2095-8137.2020.368
Citation: Cui Yang, Zhi-Lan Liu, Jun Wang, Xian-Le Bu, Yan-Jiang Wang, Yang Xiang. Parabiosis modeling: protocol, application and perspectives. Zoological Research, 2021, 42(3): 253-261. doi: 10.24272/j.issn.2095-8137.2020.368

Parabiosis modeling: protocol, application and perspectives

doi: 10.24272/j.issn.2095-8137.2020.368
Funds:  This study was supported by the National Natural Science Foundation of China (81601112, 81801090), Top Project of Youth Incubation Program of Military Medical Science and Technology (19QNP065), Sichuan Department of Science and Technology Fund (2018SZ0141, 2019YSF0213), China’s Post-doctoral Science Fund (2017M623357), and Sichuan Medical Association Fund (Q17008)
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  • Parabiosis is a surgical method of animal modeling with a long history. It has been widely used in medical research, particularly in the fields of aging, stem cells, neuroscience, and immunity in the past two decades. The protocols for parabiosis have been improved many times and are now widely accepted. However, researchers need to consider many details, from surgical operation to perioperative management, to reduce mortality and maintain the parabiosis union. Although parabiosis has certain inevitable limitations, it still has broad application prospects as an irreplaceable animal model in the medical research field.
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  • [1]
    Ajami B, Bennett JL, Krieger C, Tetzlaff W, Rossi FMV. 2007. Local self-renewal can sustain CNS microglia maintenance and function throughout adult life. Nature Neuroscience, 10(12): 1538−1543. doi: 10.1038/nn2014
    [2]
    Ajami B, Bennett JL, Krieger C, McNagny KM, Rossi FMV. 2011. Infiltrating monocytes trigger EAE progression, but do not contribute to the resident microglia pool. Nature Neuroscience, 14(9): 1142−1149. doi: 10.1038/nn.2887
    [3]
    Bert P. 1864. Experiences et considerations sur la greffe animale. Journal de l'Anatomie et de la Physiologie, 1: 69−87.
    [4]
    Brack AS, Conboy MJ, Roy S, Lee M, Kuo CJ, Keller C, et al. 2007. Increased Wnt signaling during aging alters muscle stem cell fate and increases fibrosis. Science, 317(5839): 807−810. doi: 10.1126/science.1144090
    [5]
    Bu XL, Xiang Y, Jin WS, Wang J, Shen LL, Huang ZL, et al. 2018. Blood-derived amyloid-β protein induces Alzheimer's disease pathologies. Molecular Psychiatry, 23(9): 1948−1956. doi: 10.1038/mp.2017.204
    [6]
    Castellano JM, Palner M, Li SB, Freeman GM Jr, Nguyen A, Shen B, et al. 2016. In vivo assessment of behavioral recovery and circulatory exchange in the peritoneal parabiosis model. Scientific Reports, 6: 29015. doi: 10.1038/srep29015
    [7]
    Chimoskey JE, Spielman WS, Brandt MA, Heidemann SR. 1984. Cardiac atria of BIO 14.6 hamsters are deficient in natriuretic factor. Science, 223(4638): 820−822. doi: 10.1126/science.6538050
    [8]
    Coleman DL. 2010. A historical perspective on leptin. Nature Medicine, 16(10): 1097−1099. doi: 10.1038/nm1010-1097
    [9]
    Conboy IM, Conboy MJ, Wagers AJ, Girma ER, Weissman IL, Rando TA. 2005. Rejuvenation of aged progenitor cells by exposure to a young systemic environment. Nature, 433(7027): 760−764. doi: 10.1038/nature03260
    [10]
    Conboy MJ, Conboy IM, Rando TA. 2013. Heterochronic parabiosis: historical perspective and methodological considerations for studies of aging and longevity. Aging Cell, 12(3): 525−530. doi: 10.1111/acel.12065
    [11]
    Davison J. 1966. Chimeric and ex-parabiotic frogs (Rana pipiens): specificity of tolerance. Science, 152(3726): 1250−1253. doi: 10.1126/science.152.3726.1250
    [12]
    Dick SA, Macklin JA, Nejat S, Momen A, Clemente-Casares X, Althagafi MG, et al. 2019. Self-renewing resident cardiac macrophages limit adverse remodeling following myocardial infarction. Nature Immunology, 20(1): 29−39. doi: 10.1038/s41590-018-0272-2
    [13]
    Eggel A, Wyss-Coray T. 2014. A revival of parabiosis in biomedical research. Swiss Medical Weekly, 144: w13914.
    [14]
    Finerty JC, Panos TC. 1951. Parabiosis intoxication. Experimental Biology and Medicine, 76(4): 833−835. doi: 10.3181/00379727-76-18647
    [15]
    Garzia L, Kijima N, Morrissy AS, De Antonellis P, Guerreiro-Stucklin A, Holgado BL, et al. 2018. A hematogenous route for medulloblastoma leptomeningeal metastases. Cell, 172(5): 1050−1062.e14. doi: 10.1016/j.cell.2018.01.038
    [16]
    Goldmann T, Wieghofer P, Jordão MJC, Prutek F, Hagemeyer N, Frenzel K, et al. 2016. Origin, fate and dynamics of macrophages at central nervous system interfaces. Nature Immunology, 17(7): 797−805. doi: 10.1038/ni.3423
    [17]
    Harris RB, Martin RJ. 1984. Specific depletion of body fat in parabiotic partners of tube-fed obese rats. American Journal of Physiology, 247(2): R380−R386.
    [18]
    Hervey GR. 1959. The effects of lesions in the hypothalamus in parabiotic rats. The Journal of Physiology, 145(2): 336−352. doi: 10.1113/jphysiol.1959.sp006145
    [19]
    Im SJ, Konieczny BT, Hudson WH, Masopust D, Ahmed R. 2020. PD-1+ stemlike CD8 T cells are resident in lymphoid tissues during persistent LCMV infection. Proceedings of the National Academy of Sciences of the United States of America, 117(8): 4292−4299. doi: 10.1073/pnas.1917298117
    [20]
    Kadoki M, Patil A, Thaiss CC, Brooks DJ, Pandey S, Deep D, et al. 2017. Organism-level analysis of vaccination reveals networks of protection across tissues. Cell, 171(2): 398−413.e21. doi: 10.1016/j.cell.2017.08.024
    [21]
    Kamran P, Sereti KI, Zhao P, Ali SR, Weissman IL, Ardehali R. 2013. Parabiosis in mice: a detailed protocol. Journal of Visualized Experiments, (80): 50556.
    [22]
    Karin O, Alon U. 2020. Senescent cell accumulation mechanisms inferred from parabiosis. GeroScience, doi: 10.1007/s11357-020-00286-x.
    [23]
    Katsimpardi L, Litterman NK, Schein PA, Miller CM, Loffredo FS, Wojtkiewicz GR, et al. 2014. Vascular and neurogenic rejuvenation of the aging mouse brain by young systemic factors. Science, 344(6184): 630−634. doi: 10.1126/science.1251141
    [24]
    Kiss T, Tarantini S, Csipo T, Balasubramanian P, Nyúl-Tóth Á, Yabluchanskiy A, et al. 2020. Circulating anti-geronic factors from heterochonic parabionts promote vascular rejuvenation in aged mice: transcriptional footprint of mitochondrial protection, attenuation of oxidative stress, and rescue of endothelial function by young blood. GeroScience, 42(2): 727−748. doi: 10.1007/s11357-020-00180-6
    [25]
    Liu K, Waskow C, Liu XT, Yao KH, Hoh J, Nussenzweig M. 2007. Origin of dendritic cells in peripheral lymphoid organs of mice. Nature Immunology, 8(6): 578−583. doi: 10.1038/ni1462
    [26]
    Loffredo FS, Steinhauser ML, Jay SM, Gannon J, Pancoast JR, Yalamanchi P, et al. 2013. Growth differentiation factor 11 is a circulating factor that reverses age-related cardiac hypertrophy. Cell, 153(4): 828−839. doi: 10.1016/j.cell.2013.04.015
    [27]
    Mehdipour M, Skinner C, Wong N, Lieb M, Liu C, Etienne J, et al. 2020. Rejuvenation of three germ layers tissues by exchanging old blood plasma with saline-albumin. Aging (Albany NY), 12(10): 8790−8819.
    [28]
    Middeldorp J, Lehallier B, Villeda SA, Miedema SSM, Evans E, Czirr E, et al. 2016. Preclinical assessment of young blood plasma for alzheimer disease. JAMA Neurology, 73(11): 1325−1333. doi: 10.1001/jamaneurol.2016.3185
    [29]
    Mordes JP, Rossini AA. 1981. Tumor-induced anorexia in the Wistar rat. Science, 213(4507): 565−567. doi: 10.1126/science.6941477
    [30]
    Nisbet NW. 1968. Mechanisms in early and late phases of tolerance induced by parabiosis in adult mice. Nature, 217(5125): 265−267. doi: 10.1038/217265a0
    [31]
    Perera J, Meng LP, Meng FY, Huang HC. 2013. Autoreactive thymic B cells are efficient antigen-presenting cells of cognate self-antigens for T cell negative selection. Proceedings of the National Academy of Sciences of the United States of America, 110(42): 17011−17016. doi: 10.1073/pnas.1313001110
    [32]
    Rieux M, Alpaugh M, Sciacca G, Saint-Pierre M, Masnata M, Denis HL, et al. 2020. Shedding a new light on Huntington's disease: how blood can both propagate and ameliorate disease pathology. Molecular Psychiatry, doi: 10.1038/s41380-020-0787-4.
    [33]
    Rinkevich Y, Lindau P, Ueno H, Longaker MT, Weissman IL. 2011. Germ-layer and lineage-restricted stem/progenitors regenerate the mouse digit tip. Nature, 476(7361): 409−413. doi: 10.1038/nature10346
    [34]
    Rossi FMV, Corbel SY, Merzaban JS, Carlow DA, Gossens K, Duenas J, et al. 2005. Recruitment of adult thymic progenitors is regulated by P-selectin and its ligand PSGL-1. Nature Immunology, 6(6): 626−634. doi: 10.1038/ni1203
    [35]
    Sinha M, Jang YC, Oh J, Khong D, Wu EY, Manohar R, et al. 2014. Restoring systemic GDF11 levels reverses age-related dysfunction in mouse skeletal muscle. Science, 344(6184): 649−652. doi: 10.1126/science.1251152
    [36]
    Villeda SA, Luo J, Mosher KI, Zou BD, Britschgi M, Bieri G, et al. 2011. The ageing systemic milieu negatively regulates neurogenesis and cognitive function. Nature, 477(7362): 90−94. doi: 10.1038/nature10357
    [37]
    Villeda SA, Plambeck KE, Middeldorp J, Castellano JM, Mosher KI, Luo J, et al. 2014. Young blood reverses age-related impairments in cognitive function and synaptic plasticity in mice. Nature Medicine, 20(6): 659−663. doi: 10.1038/nm.3569
    [38]
    Walker DG. 1973. Osteopetrosis cured by temporary parabiosis. Science, 180(4088): 875. doi: 10.1126/science.180.4088.875
    [39]
    Wang J, Jin WS, Bu XL, Zeng F, Huang ZL, Li WW, et al. 2018. Physiological clearance of tau in the periphery and its therapeutic potential for tauopathies. Acta Neuropathologica, 136(4): 525−536. doi: 10.1007/s00401-018-1891-2
    [40]
    Wang XF, Sun R, Hao XL, Lian ZX, Wei HM, Tian ZG. 2019. IL-17 constrains natural killer cell activity by restraining IL-15-driven cell maturation via SOCS3. Proceedings of the National Academy of Sciences of the United States of America, 116(35): 17409−17418. doi: 10.1073/pnas.1904125116
    [41]
    Xiang Y, Bu XL, Liu YH, Zhu C, Shen LL, Jiao SS, et al. 2015. Physiological amyloid-beta clearance in the periphery and its therapeutic potential for Alzheimer's disease. Acta Neuropathologica, 130(4): 487−499. doi: 10.1007/s00401-015-1477-1
    [42]
    Yousefzadeh MJ, Wilkinson JE, Hughes B, Gadela N, Ladiges WC, Vo N, et al. 2020. Heterochronic parabiosis regulates the extent of cellular senescence in multiple tissues. GeroScience, 42(3): 951−961. doi: 10.1007/s11357-020-00185-1
    [43]
    Zhang YY, Proenca R, Maffei M, Barone M, Leopold L, Friedman JM. 1994. Positional cloning of the mouse obese gene and its human homologue. Nature, 372(6505): 425−432. doi: 10.1038/372425a0
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