Transcriptomic analyses of humans and mice provide insights into depression

Hui-Juan Li; Xi Su; Lu-Wen Zhang; Chu-Yi Zhang; Lu Wang; Wen-Qiang Li; Yong-Feng Yang; Lu-Xian Lv; Ming Li; Xiao Xiao

doi:10.24272/j.issn.2095-8137.2020.174

Volume 41 Issue 6

Nov. 2020

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Article Navigation > Zoological Research > 2020 > 41(6): 632-643

Hui-Juan Li, Xi Su, Lu-Wen Zhang, Chu-Yi Zhang, Lu Wang, Wen-Qiang Li, Yong-Feng Yang, Lu-Xian Lv, Ming Li, Xiao Xiao. Transcriptomic analyses of humans and mice provide insights into depression. Zoological Research, 2020, 41(6): 632-643. doi: 10.24272/j.issn.2095-8137.2020.174

Citation:

Hui-Juan Li, Xi Su, Lu-Wen Zhang, Chu-Yi Zhang, Lu Wang, Wen-Qiang Li, Yong-Feng Yang, Lu-Xian Lv, Ming Li, Xiao Xiao. Transcriptomic analyses of humans and mice provide insights into depression. Zoological Research, 2020, 41(6): 632-643. doi: 10.24272/j.issn.2095-8137.2020.174

Citation:

Hui-Juan Li, Xi Su, Lu-Wen Zhang, Chu-Yi Zhang, Lu Wang, Wen-Qiang Li, Yong-Feng Yang, Lu-Xian Lv, Ming Li, Xiao Xiao. Transcriptomic analyses of humans and mice provide insights into depression. Zoological Research, 2020, 41(6): 632-643. doi: 10.24272/j.issn.2095-8137.2020.174

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Transcriptomic analyses of humans and mice provide insights into depression

doi: 10.24272/j.issn.2095-8137.2020.174

Hui-Juan Li^{1, 2, #},
Xi Su^{3, 4, #},
Lu-Wen Zhang^{3, 4, #},
Chu-Yi Zhang^{1, 2},
Lu Wang¹,
Wen-Qiang Li^{3, 4},
Yong-Feng Yang^{3, 4},
Lu-Xian Lv^{3, 4, 5},
Ming Li^{1, 2, 6
,
,},
Xiao Xiao^{1
,
,}

1.
Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences and Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
2.
Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming, Yunnan 650204, China
3.
Henan Mental Hospital, Second Affiliated Hospital of Xinxiang Medical University, Xinxiang, Henan 453002, China
4.
Henan Key Lab of Biological Psychiatry, International Joint Research Laboratory for Psychiatry and Neuroscience of Henan, Xinxiang Medical University, Xinxiang, Henan 453002, China
5.
Henan Province People’s Hospital, Zhengzhou, Henan 450003, China
6.
KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China

#Authors contributed equally to this work

Funds: This study was supported by the Chinese Academy of Sciences (CAS) Western Light Program and CAS Youth Innovation Promotion Association to X.X. and the CAS Pioneer Hundred Talents Program and 1000 Young Talents Program to M.L.

More Information

Corresponding author: E-mail: limingkiz@mail.kiz.ac.cn; xiaoxiao2@mail.kiz.ac.cn
Received Date: 2020-07-01
Accepted Date: 2020-09-07

Published Online: 2020-09-08

Publish Date: 2020-11-18

Abstract

Abstract

Accumulating studies have been conducted to identify risk genes and relevant biological mechanisms underlying major depressive disorder (MDD). In particular, transcriptomic analyses in brain regions engaged in cognitive and emotional processes, e.g., the dorsolateral prefrontal cortex (DLPFC), have provided essential insights. Based on three independent DLPFC RNA-seq datasets of 79 MDD patients and 75 healthy controls, we performed differential expression analyses using two alternative approaches for cross-validation. We also conducted transcriptomic analyses in mice undergoing chronic variable stress (CVS) and chronic social defeat stress (CSDS). We identified 12 differentially expressed genes (DEGs) through both analytical methods in MDD patients, the majority of which were also dysregulated in stressed mice. Notably, the mRNA level of the immediate early gene FOS (Fos proto-oncogene) was significantly decreased in both MDD patients and CVS-exposed mice, and CSDS-susceptible mice exhibited a greater reduction in Fos expression compared to resilient mice. These findings suggest the potential key roles of this gene in the pathogenesis of MDD related to stress exposure. Altered transcriptomes in the DLPFC of MDD patients might be, at least partially, the result of stress exposure, supporting that stress is a primary risk factor for MDD.
- Major depressive disorder,
- Stressed mice,
- Dorsolateral prefrontal cortex,
- Transcriptomic analysis,
- FOS

#Authors contributed equally to this work

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References(92)

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