Successful implementation of intestinal resection and anastomosis in non-human primates suggests the possibility of longitudinal intestinal research

Xue-Hui Wang; Tian-Zhang Song; Lei Li; Ren-Rong Tian; Yong-Tang Zheng

doi:10.24272/j.issn.2095-8137.2020.049

Volume 41 Issue 4

Jul. 2020

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Xue-Hui Wang, Tian-Zhang Song, Lei Li, Ren-Rong Tian, Yong-Tang Zheng. Successful implementation of intestinal resection and anastomosis in non-human primates suggests the possibility of longitudinal intestinal research. Zoological Research, 2020, 41(4): 449-454. doi: 10.24272/j.issn.2095-8137.2020.049

Citation:

Xue-Hui Wang, Tian-Zhang Song, Lei Li, Ren-Rong Tian, Yong-Tang Zheng. Successful implementation of intestinal resection and anastomosis in non-human primates suggests the possibility of longitudinal intestinal research. Zoological Research, 2020, 41(4): 449-454. doi: 10.24272/j.issn.2095-8137.2020.049

Citation:

Xue-Hui Wang, Tian-Zhang Song, Lei Li, Ren-Rong Tian, Yong-Tang Zheng. Successful implementation of intestinal resection and anastomosis in non-human primates suggests the possibility of longitudinal intestinal research. Zoological Research, 2020, 41(4): 449-454. doi: 10.24272/j.issn.2095-8137.2020.049

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Successful implementation of intestinal resection and anastomosis in non-human primates suggests the possibility of longitudinal intestinal research

doi: 10.24272/j.issn.2095-8137.2020.049

1.
School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230026, China
2.
Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences, KIZ-CUHK Joint Laboratory of Bioresources and Molecular Research in Common Diseases, Center for Biosafety Mega-Science, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China
3.
Dongfang Animal Hospital, Kunming, Yunnan 650228, China

Funds: This work was partly supported by the National Natural Science Foundation of China (U1802284, 81671627, 81601808) and 13th Five-Year Key Scientific and Technological Program of China (2017ZX10202102-001-005, 2018ZX10301406-003, 2018ZX10301101-002)

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Corresponding author: E-mail: zhengyt@mail.kiz.ac.cn
Received Date: 2020-01-17
Accepted Date: 2020-05-06

Published Online: 2020-06-01

Publish Date: 2020-07-18

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Author Correction: Successful implementation of intestinal resection and anastomosis in non-human primates suggests the possibility of longitudinal intestinal research

Xue-Hui Wang, Tian-Zhang Song, Lei Li, Ren-Rong Tian, Yong-Tang Zheng
2021, 42(2): 1-2. doi: 10.24272/j.issn.2095-8137.2020.383

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Abstract

Abstract

Intestinal biopsy is a basic experimental method for studying pathological changes in the intestinal tract during human immunodeficiency virus (HIV) infection. In this study, jejunal resection and anastomosis were successfully performed in 12 Chinese rhesus macaques (Macaca mulatta). The sampled gut tissues were then examined by hematoxylin and eosin (H&E) staining, electron microscopy, flow cytometry, immunofluorescence detection, and RNA quality analysis to ensure suitability for histological, physiological, pathological, and immunological detection, as well as mechanistic analysis at the cellular and molecular level. Importantly, the surgery did not affect the ratio or number of immune cells in peripheral blood or the concentration of lipids, proteins, and vitamins in plasma, which are important indicators of nutritional status. Our results thus indicated that jejunal resection and anastomosis are feasible, and that immune homeostasis and intestinal barrier integrity are not altered by surgery. All macaques recovered well (except for one), with no postoperative complications. Therefore, this animal surgery may be applicable for longitudinal intestinal research related to diseases such as acquired immunodeficiency syndrome (AIDS).
- Jejunal resection and anastomosis,
- Rhesus macaques,
- Biopsy, Surgery,
- Surgery,
- Nursing care

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References(22)

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