Volume 41 Issue 3
May  2020
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Zi-Qin Jiang, Han-Yu Wu, Jing Tian, Ning Li, Xiao-Xiang Hu. Targeting lentiviral vectors to primordial germ cells (PGCs): An efficient strategy for generating transgenic chickens. Zoological Research, 2020, 41(3): 281-291. doi: 10.24272/j.issn.2095-8137.2020.032
Citation: Zi-Qin Jiang, Han-Yu Wu, Jing Tian, Ning Li, Xiao-Xiang Hu. Targeting lentiviral vectors to primordial germ cells (PGCs): An efficient strategy for generating transgenic chickens. Zoological Research, 2020, 41(3): 281-291. doi: 10.24272/j.issn.2095-8137.2020.032

Targeting lentiviral vectors to primordial germ cells (PGCs): An efficient strategy for generating transgenic chickens

doi: 10.24272/j.issn.2095-8137.2020.032
#Authors contributed equally to this work
Funds:  This study was supported by the National Transgenic Breeding Project of China (2016ZX08009003006), National Natural Science Foundation of China (31672411) and Discipline Innovative Engineering Plan (B12008)
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  • Corresponding author: E-mail: huxx@cau.edu.cn
  • Received Date: 2019-12-11
  • Accepted Date: 2020-04-03
  • Published Online: 2020-04-18
  • Publish Date: 2020-05-18
  • Recent advances in avian transgenic studies highlight the possibility of utilizing lentiviral vectors as tools to generate transgenic chickens. However, low rates of gonadal chimerism and germ line transmission efficiency still limit the broad usage of this method in creating transgenic chickens. In this study, we implemented a simple strategy using modified lentiviral vectors targeted to chicken primordial germ cells (PGCs) to generate transgenic chickens. The lentiviral vectors were pseudotyped with a modified Sindbis virus envelope protein (termed M168) and conjugated with an antibody specific to PGC membrane proteins. We demonstrated that these optimized M168-pseudotyped lentiviral vectors conjugated with SSEA4 antibodies successfully targeted transduction of PGCs in vitro and in vivo. Compared with the control, 50.0%–66.7% of chicken embryos expressed green fluorescent protein (GFP) in gonads transduced by the M168-pseudotyped lentivirus. This improved the targeted transduction efficiency by 30.0%–46.7%. Efficient chimerism of exogenous genes was also observed. This targeting technology could improve the efficiency of germ line transmission and provide greater opportunities for transgenic poultry studies.

  • #Authors contributed equally to this work
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