Co-localization of two-color rAAV2-retro confirms the dispersion characteristics of efferent projections of mitral cells in mouse accessory olfactory bulb

Ning Zheng; Zhi-Zhong Wang; Song-Wei Wang; Fang-Jia Yang; Xu-Tao Zhu; Chen Lu; Anne Manyande; Xiao-Ping Rao; Fu-Qiang Xu

doi:10.24272/j.issn.2095-8137.2020.020

Volume 41 Issue 2

Mar. 2020

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Article Navigation > Zoological Research > 2020 > 41(2): 148-156

Ning Zheng, Zhi-Zhong Wang, Song-Wei Wang, Fang-Jia Yang, Xu-Tao Zhu, Chen Lu, Anne Manyande, Xiao-Ping Rao, Fu-Qiang Xu. Co-localization of two-color rAAV2-retro confirms the dispersion characteristics of efferent projections of mitral cells in mouse accessory olfactory bulb. Zoological Research, 2020, 41(2): 148-156. doi: 10.24272/j.issn.2095-8137.2020.020

Citation:

Ning Zheng, Zhi-Zhong Wang, Song-Wei Wang, Fang-Jia Yang, Xu-Tao Zhu, Chen Lu, Anne Manyande, Xiao-Ping Rao, Fu-Qiang Xu. Co-localization of two-color rAAV2-retro confirms the dispersion characteristics of efferent projections of mitral cells in mouse accessory olfactory bulb. Zoological Research, 2020, 41(2): 148-156. doi: 10.24272/j.issn.2095-8137.2020.020

Citation:

Ning Zheng, Zhi-Zhong Wang, Song-Wei Wang, Fang-Jia Yang, Xu-Tao Zhu, Chen Lu, Anne Manyande, Xiao-Ping Rao, Fu-Qiang Xu. Co-localization of two-color rAAV2-retro confirms the dispersion characteristics of efferent projections of mitral cells in mouse accessory olfactory bulb. Zoological Research, 2020, 41(2): 148-156. doi: 10.24272/j.issn.2095-8137.2020.020

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Co-localization of two-color rAAV2-retro confirms the dispersion characteristics of efferent projections of mitral cells in mouse accessory olfactory bulb

doi: 10.24272/j.issn.2095-8137.2020.020

1.
Center of Brain Science, State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Key Laboratory of Magnetic Resonance in Biological Systems, Wuhan Institute of Physics and Mathematics, Innovation Academy for Precision Measurement Science and Technology, Chinese Academy of Sciences, Wuhan, Hubei 430071, China
2.
University of the Chinese Academy of Sciences, Beijing 100049, China
3.
Department of Automation, School of Electrical Engineering, Zhengzhou University, Zhengzhou, Henan 450001, China
4.
School of Life Science, Wuhan University, Wuhan, Hubei 430072, China
5.
School of Human and Social Sciences, University of West London, Middlesex TW89GA, UK
6.
Divisions of Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Wuhan, Hubei 430074, China

#Authors contributed equally to this work

Funds: This work was supported by the National Natural Science Foundation of China (31400946, 31771156, 91632303/H09, 91732304 and 31830035) and Strategic Priority Research Program of the Chinese Academy of Sciences (XDB32030200)

More Information

Corresponding author: E-mail: raoxiaoping0902@wipm.ac.cn; fuqiang.xu@wipm.ac.cn
Received Date: 2019-09-18
Publish Date: 2020-03-01

Abstract

Abstract

The accessory olfactory bulb (AOB), located at the posterior dorsal aspect of the main olfactory bulb (MOB), is the first brain relay of the accessory olfactory system (AOS), which can parallelly detect and process volatile and nonvolatile social chemosignals and mediate different sexual and social behaviors with the main olfactory system (MOS). However, due to its anatomical location and absence of specific markers, there is a lack of research on the internal and external neural circuits of the AOB. This issue was addressed by single-color labeling and fluorescent double labeling using retrograde rAAVs injected into the bed nucleus of the stria terminalis (BST), anterior cortical amygdalar area (ACo), medial amygdaloid nucleus (MeA), and posteromedial cortical amygdaloid area (PMCo) in mice. We demonstrated the effectiveness of this AOB projection neuron labeling method and showed that the mitral cells of the AOB exhibited efferent projection dispersion characteristics similar to those of the MOB. Moreover, there were significant differences in the number of neurons projected to different brain regions, which indicated that each mitral cell in the AOB could project to a different number of neurons in different cortices. These results provide a circuitry basis to help understand the mechanism by which pheromone information is encoded and decoded in the AOS.
- Accessory olfactory bulb,
- Efferent projections,
- Retrograde rAAVs,
- Projection neuron labeling,
- Dispersion characteristics,
- Circuitry basis

#Authors contributed equally to this work

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References(32)

References

[1]	Ackels T, Drose DR, Spehr M. 2016. In-depth physiological analysis of defined cell populations in acute tissue slices of the mouse vomeronasal organ. Journal of Visuallized Experiments, 115: e54517.
[2]	Breer H, Fleischer J, Strotmann J. 2006. The sense of smell: multiple olfactory subsystems. Cellular and Molecular Life Sciences, 63: 1465−1475. doi: 10.1007/s00018-006-6108-5
[3]	Doving KB, Trotier D. 1998. Structure and function of the vomeronasal organ. Journal of Experimental Biologyl, 201: 2913−2925.
[4]	Dulac C, Torello AT. 2003. Molecular detection of pheromone signals in mammals: from genes to behaviour. Nature Reviews Neuroscience, 4: 551−562. doi: 10.1038/nrn1140
[5]	Dulac C, Wagner S. 2006. Genetic analysis of brain circuits underlying pheromone signaling. Annual Review of Genetics, 40: 449−467. doi: 10.1146/annurev.genet.39.073003.093937
[6]	Ghosh S, Larson SD, Hefzi H, Marnoy Z, Cutforth T, Dokka K, Baldwin KK. 2011. Sensory maps in the olfactory cortex defined by long-range viral tracing of single neurons. Nature, 472: 217−220. doi: 10.1038/nature09945
[7]	Halpern M, Martinez-Marcos A. 2003. Structure and function of the vomeronasal system: an update. Progress in Neurobiology, 70(3): 245−318. doi: 10.1016/S0301-0082(03)00103-5
[8]	Holy TE. 2018. The accessory olfactory system: Innately specialized or microcosm of mammalian circuitry?. Annual review of neuroscience, 41: 501−525. doi: 10.1146/annurev-neuro-080317-061916
[9]	Kang N, Baum MJ, Cherry JA. 2009. A direct main olfactory bulb projection to the 'vomeronasal' amygdala in female mice selectively responds to volatile pheromones from males. The European Journal of Neuroscience, 29(3): 624−634. doi: 10.1111/j.1460-9568.2009.06638.x
[10]	Krieger J, Schmitt A, Lobel D, Gudermann T, Schultz G, Breer H, Boekhoff I. 1999. Selective activation of G protein subtypes in the vomeronasal organ upon stimulation with urine-derived compounds. The Journal of Biological Chemistry, 274(8): 4655−4662. doi: 10.1074/jbc.274.8.4655
[11]	Larriva-Sahd J. 2008. The accessory olfactory bulb in the adult rat: a cytological study of its cell types, neuropil, neuronal modules, and interactions with the main olfactory system. The Journal of Comparative Neurology, 510(3): 309−350. doi: 10.1002/cne.21790
[12]	Leinders-Zufall T, Brennan P, Widmayer P, S PC, Maul-Pavicic A, Jager M, Li XH, Breer H, Zufall F, Boehm T. 2004. MHC class I peptides as chemosensory signals in the vomeronasal organ. Science, 306(5698): 1033−1037. doi: 10.1126/science.1102818
[13]	Leinders-Zufall T, Lane AP, Puche AC, Ma W, Novotny MV, Shipley MT, Zufall F. 2000. Ultrasensitive pheromone detection by mammalian vomeronasal neurons. Nature, 405: 792−796. doi: 10.1038/35015572
[14]	Marking S, Krosnowski K, Ogura T, Lin W. 2017. Dichotomous distribution of putative cholinergic interneurons in mouse accessory olfactory bulb. Frontiers in Neuroanatomy, 11: 10.
[15]	Miyamichi K, Amat F, Moussavi F, Wang C, Wickersham I, Wall NR, Taniguchi H, Tasic B, Huang ZJ, He Z, Callaway EM, Horowitz MA, Luo L. 2011. Cortical representations of olfactory input by trans-synaptic tracing. Nature, 472: 191−196. doi: 10.1038/nature09714
[16]	Mohedano-Moriano A, Pro-Sistiaga P, Ubeda-Banon I, Crespo C, Insausti R, Martinez-Marcos A. 2007. Segregated pathways to the vomeronasal amygdala: differential projections from the anterior and posterior divisions of the accessory olfactory bulb. The European Journal of Neuroscience, 25(7): 2065−2080. doi: 10.1111/j.1460-9568.2007.05472.x
[17]	Mohrhardt J, Nagel M, Fleck D, Ben-Shaul Y, Spehr M. 2018. Signal detection and coding in the accessory olfactory system. Chemical Senses, 43(9): 667−695. doi: 10.1093/chemse/bjy061
[18]	Moriya-Ito K, Endoh K, Fujiwara-Tsukamoto Y, Ichikawa M. 2013. Three-dimensional reconstruction of electron micrographs reveals intrabulbar circuit differences between accessory and main olfactory bulbs. Frontiers in Neuroanatomy, 7: 5.
[19]	Nagayama S, Enerva A, Fletcher ML, Masurkar AV, Igarashi KM, Mori K, Chen WR. 2010. Differential axonal projection of mitral and tufted cells in the mouse main olfactory system. Frontiers in Neural Circuits, 4: 120.
[20]	Perez-Gomez A, Bleymehl K, Stein B, Pyrski M, Birnbaumer L, Munger SD, Leinders-Zufall T, Zufall F, Chamero P. 2015. Innate predator odor aversion driven by parallel olfactory subsystems that converge in the ventromedial hypothalamus. Current Biology, 25(10): 1340−1346. doi: 10.1016/j.cub.2015.03.026
[21]	Riviere S, Challet L, Fluegge D, Spehr M, Rodriguez I. 2009. Formyl peptide receptor-like proteins are a novel family of vomeronasal chemosensors. Nature, 459: 574−577. doi: 10.1038/nature08029
[22]	Sosulski DL, Bloom ML, Cutforth T, Axel R, Datta SR. 2011. Distinct representations of olfactory information in different cortical centres. Nature, 472: 213−216. doi: 10.1038/nature09868
[23]	Spehr M, Spehr J, Ukhanov K, Kelliher KR, Leinders-Zufall T, Zufall F. 2006. Parallel processing of social signals by the mammalian main and accessory olfactory systems. Cellular Molecular Life Sciences, 63: 1476−1484. doi: 10.1007/s00018-006-6109-4
[24]	Su CY, Menuz K, Carlson JR. 2009. Olfactory perception: receptors, cells, and circuits. Cell, 139: 45−59. doi: 10.1016/j.cell.2009.09.015
[25]	Takami S, Graziadei PP. 1991. Light microscopic Golgi study of mitral/tufted cells in the accessory olfactory bulb of the adult rat. The Journal of Comparative Neurology, 311(1): 65−83. doi: 10.1002/cne.903110106
[26]	Urban NN, Castro JB. 2005. Tuft calcium spikes in accessory olfactory bulb mitral cells. The Journal of Neuroscience: the Official Journal of the Society for Neuroscience, 25(20): 5024−5028. doi: 10.1523/JNEUROSCI.0297-05.2005
[27]	Vargas-Barroso V, Ordaz-Sanchez B, Pena-Ortega F, Larriva-Sahd JA. 2015. Electrophysiological evidence for a direct link between the main and accessory olfactory bulbs in the adult rat. Frontiers in Neuroscience, 9: 518.
[28]	von Campenhausen H, Mori K. 2000. Convergence of segregated pheromonal pathways from the accessory olfactory bulb to the cortex in the mouse. The European Journal of Neuroscience, 12(1): 33−46. doi: 10.1046/j.1460-9568.2000.00879.x
[29]	Yokosuka M. 2012. Histological properties of the glomerular layer in the mouse accessory olfactory bulb. Experimental Animals, 61(1): 13−24. doi: 10.1538/expanim.61.13
[30]	Yoles-Frenkel M, Kahan A, Ben-Shaul Y. 2018. Temporal response properties of accessory olfactory bulb neurons: limitations and opportunities for decoding. The Journal of Neuroscience, 38(21): 4957−4976. doi: 10.1523/JNEUROSCI.2091-17.2018
[31]	Yonekura J, Yokoi M. 2008. Conditional genetic labeling of mitral cells of the mouse accessory olfactory bulb to visualize the organization of their apical dendritic tufts. Molecular and Cellular Neuroscience, 37(4): 708−718. doi: 10.1016/j.mcn.2007.12.016
[32]	Zhu X, Lin K, Liu Q, Yue X, Mi H, Huang X, He X, Wu R, Zheng D, Wei D, Jia L, Wang W, Manyande A, Wand J, Zhang Z, Xu F. 2020. Rabies virus pseudotyped with CVS-N2C glycoprotein as a powerful tool for retrograde neuronal network tracing. Neuroscience Bulletin, 36(3): 202−216. doi: 10.1007/s12264-019-00423-3