1984 Vol. 5, No. 4

Display Method:
Cell fusion between mouse myeloma NS-1 and mouse spleen cells was induced by polyethylene glycol (PEG).Hyrid cells were successfully obtained by HAT medium.The hybridcell population were examined 30,50,70 and 90 days after fusion,The G-,C- banding and the frequencies of chromosomal aberrations in parental and hybrid cells were then compared.
An investigation on the isozyme patterns of esterase and malic dehydrogenase of Periplaneta japonica,Periplaneta fuliginosa,Blattella germanica and Periplaneta americana was done.Though all revealed 6-7 bands,yet all these 4 indoor species show significant differences in the Rf value of esterase isozymes.Malic dehydrogenase gives 3 or 4 bands,showing some species-difference,but not so obvious as seen in esterase.Blattella germanica is much more different from the rest 3 species.The significance of these findings needs further elucidation.
In this paper,a new species of Lithodidae is reported.The type specimens are deposited in the Department of Biology,Hangzhou University and the Donghai Fisheries Research Institute.Diagnosis of the new species is given as below.
A great many specimens of some nemachilus fishes have been studied and their taxonomic problems are cleared up as follows.N.kungessanus Kessler is far different from N.dorsalis in the shape of air-bladder,intestine and vertebrate counts.They should not be merged into one taxon under the name of N.dorsalis,N.kungessanus can be subdivided into two subspecies,I.e.,N.k.kungessanus Kessler,and N.korientalis with elongatus as a synonym.N.papilloso-labiatus is well different from N.strauchii in a series of characters.Both are considered to be valid monotypic species.The formerly used diagnoses of N.wuweiensis are discovered not effectively enough to differentiate from the relative species N.scleropterus and,therefore,are revised.Based on their morphological differences and sympatric distribution in both river systems of Shule He and Ruo Shui of the northern Gansu Province,N.leptosoma and N.brevicauda formerly treated as two subspecies of N.stoliczkae are raised to independent species taxa.
In this article we report the that technique of sister-chromatid exchange (SCE) of peripheral lymphocyte of Bufo bufo gargarizans was used for environmental monitoring.
Categories of canine lymphocytes forming rosettes with guinea pig erythrocytes (E-RFC) were studied.The incubation of canine lymphocytes at 37℃ increased E-rosette formation.The percentages of Sig positive cells in E-RFC were similar to those in total lymphocytes.In mixed E-EAC (guinea pig erythrocytes-sheep erythrocytes coated with antibody and complement) rosette assays,a portion of canine lymphocytes having both E and complement receptors has been detected.No significant difference was found in the mean percentages of E-RFC between total lymphocytes and EAC-rosetting ones.On the basis of these results we assume that the E receptors are present on a part of T cells and also on some B cells.Thus,the E-rosette assay cannot be used for detection of canine T cells.
According to the analysis on light chain pattern of single fibers,ther were one third of fibers in rat soleus muscle which had a distinct staining band at the position of LC3,while the remaining fibers did not show such band by present method due to its minute quantity.No matter whether of not LC3 appeared,the electrophoretic patterns of fiber proteins between Sol and EDL were completely different,including the differences of molecular weights of LC1,LC2 and the distance travelled along the gel between LC1 and tropomyosin of fibers of the two muscles.Experiments have been performed to see if LC3 exists in each fiber of soleus muscle.We deduced it by analyzing randomly three fibers in one electrophoretic gel to show the chance of appearance of LC3 by simple probability calculation and a great exceed (89%) over the "theoretical" value (67%) in probability was achieved.For the same purpose,we also analyzed guinea pig soleus which was known to have pure slow fibers,and conform to that,100% of fibers tested contained LC3;and finally 56% of cat soleus fibers was found to contain LC3 whose appearnce were clearly related to their intensity of protein staining on electrophoretic gels.Attempts have also been made to explore the corelation between light chain characteristics and ATPase activity of fibers.Preliminary results indicated that there were no such parallel relationship.
During June-August,1980,the author examined the growth,development and energy intake of Upland Buzzard nestling (Buteo hemilasius) which were handreared,collected from Haibei alpine meadoe.Results obtained may be summarizad as follows:Nestling period is about 50 days.Feather begins to grow at the 7th day of age,completed by the 50th day.Nestlings open their eyes and chirp soon after hatching.At the 23rd day,they can grasp food with talon and stand up.They tear up food effectively and even fight during the 7th week.Growth curves were fitted with "logistic" equation.Growth rate is 0.156;t10-90 is 28.2 days.The mean caloric intake are 0.386±0.166 Kcal/g.wt/day.The relationship between the daily energy intake and growth of body weight is an exponential fuction.This relationship may be expressed by the following equation:DEI=18.239W-0.578.
[125]Ⅰ-labelled "defibrinase" had been prepared by oxidant-chloroamine T.Drug is given in different ways:Subcutaneous,muscle,intravenous injection,the cases of distribution and excretion in different times in internal organs and in blood of rats,mice,and rabbits had been observed.It had been found that the time which [125]Ⅰ-labelled "defibrinase" needed to eliminate half of its radioactive dose from blood in the animals was three of four hours.The times when radioactive distribution reached to its highest point by different ways of giving drugs in each internal organs of animals were varied;six hours for subcutaneous injection,three hours and one hours for muscl and intravenous injection.Compared with other parts of the accumulation of the [125]Ⅰ-labelled "defibrinase" in kidney is the highest.The large quantity of by-products of "defibrinase metabolids" is eliminated from urine by kidney,the rest of it by excrements.