Volume 22 Issue 2
Mar.  2001
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WANG Lai-Yuan, WANG Jin-Xing, ZHAO Xiao-Fan, WANG Lai-Cheng, KANG Cui-Jie. Construction of cDNA Library of House Fly (Musca domestic). Zoological Research, 2001, 22(2): 159-162.
Citation: WANG Lai-Yuan, WANG Jin-Xing, ZHAO Xiao-Fan, WANG Lai-Cheng, KANG Cui-Jie. Construction of cDNA Library of House Fly (Musca domestic). Zoological Research, 2001, 22(2): 159-162.

Construction of cDNA Library of House Fly (Musca domestic)

  • Received Date: 1900-01-01
  • Rev Recd Date: 1900-01-01
  • Publish Date: 2001-04-22
  • Total RNA was separated from the abdomen of house fly,after which had been challenged for 24 hours with E.coli and Staphylococcus aureus.Then,the mR NA was purified from it.After that,ds-cDNA was synthesized by reverse transcriptase from mRNA,and small cDNA which was lower than 400 bp was removed by Sep harose CL-4B spun column.After EocRⅠ/NotⅠAdaptors were added to cDNA,those that werent ligated to cDNA were removed by another Sepharose CL-4B spun column.ThecDNA was inserted into λgtll,the recombined vector packaged in vitro,infected a host strain Y1090.Thus,the cDNA library was co structed.The titer of the newly constructed cDNA library was 3.46×10[5] pfu/mL,and its recombination rate was 99.6%.The library would provide basis for t he cloning of the antimicrobial peptide genes of house fly.
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