ZHONG Shu-rong, JIN Yang, LI Dong-sheng, WANG Wan-yu, XIONG Yu-liang. Molecular Cloning and Sequence Analyses of cDNAs Encoding Seven C-type Lectin-like Protein Subunits from Daboia russellii siamensis. Zoological Research, 2005, 26(4): 337-343.
Citation:
ZHONG Shu-rong, JIN Yang, LI Dong-sheng, WANG Wan-yu, XIONG Yu-liang. Molecular Cloning and Sequence Analyses of cDNAs Encoding Seven C-type Lectin-like Protein Subunits from Daboia russellii siamensis. Zoological Research, 2005, 26(4): 337-343.
ZHONG Shu-rong, JIN Yang, LI Dong-sheng, WANG Wan-yu, XIONG Yu-liang. Molecular Cloning and Sequence Analyses of cDNAs Encoding Seven C-type Lectin-like Protein Subunits from Daboia russellii siamensis. Zoological Research, 2005, 26(4): 337-343.
Citation:
ZHONG Shu-rong, JIN Yang, LI Dong-sheng, WANG Wan-yu, XIONG Yu-liang. Molecular Cloning and Sequence Analyses of cDNAs Encoding Seven C-type Lectin-like Protein Subunits from Daboia russellii siamensis. Zoological Research, 2005, 26(4): 337-343.
Department of Animal Toxinology, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming 650223, China 2. Graduate School of the Chinese Academy of Sciences, Beijing 100039, China
Total mRNA was extracted from a venom gland of snake Daboia russellii siamensis following the manufacturers protocol of the PolyATtract System 1000 kit purchased from Promega Biotech. cDNAs encoding C-type lectins were amplified by RT-PCR and subcloned into a pMD18-T vector. Fourteen positive clones were selected for nucleotide sequencing and seven cDNAs encoding various snake venom C-type lectin-like protein precursors, designated as DRS-L1, DRS-L2, DRS-L3, DRS-L4 DRS-L5, DRS-L6 and DRS-L7, were obtained. Amino acid sequences of these proteins were deduced and each contains a carbohydrate recognition domain. Of all the deduced protein sequences, only DRS-L1 seemed to represent a closer sequence similarity to α subunits of other known snake venom C-type lectin-like proteins using the BLAST program. Homology comparison combined with analysis of cysteine position indicate that DRS-L1 and DRS-L2 are probably the light chain LC2 and LC1 of factor Ⅹ activator from Daboia russellii siamensis venom, respectively. DRS-L3 and DRS-L4 might be the β subunits of higher molecular weight C-type lectin-like proteins while DRS-L5 and DRS-L6 might be β subunits of lower molecular weight C-type lectin-like proteins. DRS-L7 might be the β subunit of a platelet membrane glycoprotein Ib-binding protein similar to echicetin.