Column Chromatography Fractionation of Ophiophagus Hannan Venom and Study of Neurotoxic Peaks

We fractionated Chinese Guangxi Ophiophagus hannah venom by CM-Sephadex C-25 column chromatography.Linear gradient elution was carried out with NaCl.Seventeen protein peaks were obtained.Their toxicity and physiological activities were estimated.Of the seven toxic peaks,six had the effect of blockading the transmission of neuromuscle juncture.The other one was much less toxic.It didnt show such effect with the same dose.