棉蚜2个乙酰胆碱酯酶cDNA片段的基因克隆与序列分析

Cloning and Sequencing of Two Acetylcholinesterase cDNA Fragments from Cotton Aphid,Aphis gossypii Glover

摘要: 采用RT-PCR技术，利用简并引物从棉蚜(Aphis gossypii Glover)中克隆出2个乙酰胆碱酯酶基因的cDNA片段，Ag.ace1和Ag.ace2。Ag.ace1基因的cDNA片段为282 bp，编码94个氨基酸；Ag.ace2基因的cDNA片段为264 bp，编码88个氨基酸。扩增获得的2个乙酰胆碱酯酶基因cDNA片段所编码的氨基酸序列均与其他昆虫的乙酰胆碱酯酶基因有很高的同源性。首次从一种昆虫中克隆出2个乙酰胆碱酯酶基因片段，为同一种昆虫中存在多个乙酰胆碱酯酶基因的假设提供了直接的分子生物学证据。

Abstract: Two acetylcholinesterase (AChE) genes cDNA fragments,Ag.acel and Ag.ace2,have been cloned from cotton aphid,Aphis gossypii Glover using degenerate primers with RT-PCR technique.Ag.acel gene cDNA fragment is of 282 bp encoding 94 amino acids,and Ag.ace2 gene cDNA fragment is of 264 bp encoding 88 amino acids.Both two putative AChE genes cDNA fragments share numerous similarities with those cloned from other insects.This is the first report of two AChE cDNA fragment sequences in the insect species,which provided the direct evidence of multiple AChE existence in insects.