Abstract: DNAs of Sarcocystis hominis-like cyst from the nature infected water buffalo (Bubalus bubalis),Sarcocystis hominis from cattle (Bos taurus),and Sarcocystis suihominis from swine (Sus scrofa) were extracted respectively.The 18S rRNA genes PCR productions of each isolate were directly sequenced.The cysts from both cattle and water buffalo sources showed a high homology within the compared 863 bp 18S rRNA gene sequences,which contain 4 main varied regions.According to the analysis,the 4 isolates both from cattle and water buffaloes probably are the same species,Sarcocystis hominis.And water buffalo can also serve as the intermediate host for S.hominis besides cattle in south China.