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王德寿, 林浩然, 蒲德永, 张耀光, 张其忠 等.. 1998: 注射促黄体素释放激素类似物和地欧酮诱导大鳍Hu和长吻Wei排卵的研究. 动物学研究, 19(3): 191-196.
引用本文: 王德寿, 林浩然, 蒲德永, 张耀光, 张其忠 等.. 1998: 注射促黄体素释放激素类似物和地欧酮诱导大鳍Hu和长吻Wei排卵的研究. 动物学研究, 19(3): 191-196.
WANG De-shou, LIN Hao-ran, PU De-yong, ZHANG Yao-guang, ZHANG Qi-zhong et al.. 1998. Domperidone-Lhrh-A-Induced Ovulation of The Bagrid Catfish,Mystus macropterus and Leiocassis longirostris. Zoological Research, 19(3): 191-196.
Citation: WANG De-shou, LIN Hao-ran, PU De-yong, ZHANG Yao-guang, ZHANG Qi-zhong et al.. 1998. Domperidone-Lhrh-A-Induced Ovulation of The Bagrid Catfish,Mystus macropterus and Leiocassis longirostris. Zoological Research, 19(3): 191-196.

注射促黄体素释放激素类似物和地欧酮诱导大鳍Hu和长吻Wei排卵的研究

Domperidone-Lhrh-A-Induced Ovulation of The Bagrid Catfish,Mystus macropterus and Leiocassis longirostris

  • 摘要: 对于性成熟的大鳍鲭Mystus macropterus(Bleeker)野生鱼,单独注射多巴胺的抑制剂地欧酮(DOM)不能影响血清促性腺激素(GTH)水平,也不能诱导排卵;单独注射类似物LHRH-A,虽能使血清GTH水平显著升高,但仅产生较低的排卵率;而当DOM与LHRH-A结合注射却显著增强LHRH-A促进血清GTH水平升高的作用,并诱导出较高的排卵率。对性成熟的长吻鲈Leiocassis longirostris Gunther野生鱼,使用LHRH-A+DOM作2次注射诱导排卵的效果也与注射 LHRH-A加脑垂体这一传统诱导排卵方法相似。Linpe方法(LHRH-A+DOM,作1次或2次注射)避免了采集、保存脑垂体不便给生产带来的麻烦,在鲩科鱼类的人工繁殖中,具有较高的推广价值。

     

    Abstract: To sexually matured feral catfish,Mystus macropterus (Bleeker),injection of domperidone (DOM),a dopamine antagonist,failed to stimulate an increase in serum gonadotropin (GTH) level and failed to induce ovulation.Injection of LHRH-A alone had an effect on stimulating an increase in serum GTH levels,but resulted in a low ovulation rate.Injection of DOM plus LHRH-A resulted in significantly higher serum GTH levels than injection of LHRH-A alone,and resulted in a significantly higher ovulatory response.To sexually matured feral catfish,Leiocassis longirostris Gunther,injection of LHRH-A plus DOM had the same effect as LHRH-A plus silver carp pituitary suspension (SCPS),which had been used to induce ovulation of the bagrid catfishes for years,in inducing ovulation.Linpe method (LHRH-A plus DOM,given in single or two injections) avoided the truble of collecting and storing the pituitary and should be popularized in the artificial propagation of the bagrids in aquaculture.

     

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