家蚕抗菌肽对癌细胞的杀伤作用及其超微结构的观察

Study on The Killing Action of The Antibacterial Peptides on Cancer Cells and Their Influence on the Ultrastructure

摘要: 从家蚕蛹血淋巴中分离纯化的抗菌肽β组分对体外培养的癌细胞有选择性杀伤作用，而对正常人B淋巴细胞无不良影响。抗菌肽与巨噬细胞淋巴瘤细胞U937体外培养一段时间后，用扫描和透射电镜观察细胞膜和细胞器的变化，发现细胞膜和核被膜局部溶解，线粒体肿胀、发空，胞内物质大量外泄，从而导致细胞死亡。

Abstract: The β-component of antibacterial peptide purified from the haemolymph of silkworm Bombyx mori.pupae induced by E.coli.can kill the cancer cells cultured in vitro selectively,but has no effect on the B-leukocytes of normal human.The ultrastructure of the U937 cells treated with the antibacterial peptides was observed with an the electron microscope.It was found that the cancer cells plasma membrane and nuclear envelope were broken,mitochondrion was swollen and intracellular substances were leaked out,and the cells died soon.