林麝微卫星座位的分离和鉴定(英文)

Isolation and Characterization of Microsatellite DNA Markers from Forest Musk Deer (Moschus berezovskii)(in English)

摘要: 利用改进的磁珠富集法从林麝(Moschus berezovskii)的基因组中分离到10个新的(AC)n重复微卫星座位，并分析其在24个林麝个体(取样于中国四川金凤山群体)中的多态性。其中，5个位点在24个林麝个体中具有4—13个等位基因，观察杂合度和期望杂合度分别是0.429—0.957和0.587—0.902，平均多态信息含量是0.730。表明这5个微卫星位点具有高度的多态性，可以用于林麝遗传多样性研究，对林麝的保护具有很重要的意义。

Abstract: In this report, we describe the isolation and characterization of microsatellite loci for forest musk deer (Moschus berezovskii) through an improved enrichment protocol. Five new markers were isolated from the genomic DNA of forest musk deer and showed high polymorphism with 4-13 alleles in 24 sampled individuals from the population of Jinfeng Mountain, Sichuan Province, China. The observed and expected heterozygosities were from 0.429-0.957 and 0.587-0.902, respectively. The average polymorphism information content (PIC) value in these five loci was 0.730. This suggests that the five microsatellite loci are a valuable tool for further studies about forest musk deer.