Abstract: A population of one same species could adapt changing environmental facts of its own,including food sources, breeding sites, microclimate of habitat, which will undoubtedly cause transformation of behavior, habits, external and internal characterstics, until form diversified population. Some of these populations would arise to a subdivision into subspecies or even independent species.Megophrys lateralis was widely distributed in many countries in southeastern Asia. Certainly, there are a lot of the isolates of this species in Indo-Malaya region. In the course of the origin, of course, must relate to the similarity between populations, As indicated by the dim clustering analysis, we found the transfomation of characters are continuous, in three populations of M.lateralis, none of the characteristic reached subspecies level. However, M. glandulosa which is not a valid species. Because of using unstable characters, such as fronto-parietal separated from squamosal by prootica, unmeasurable characteristics, for example, swelling of posterior of temporal fold and tubercles in size etc. We propose that all of utilize unmeasured characters should determine in size and to calculate ratio wiht a length of part of bogy, In such case, one can avoid the fault result.The similarity between populations of M. lateralis show that the population of Jingdong is similar to that of Tengchun, both are a little different from that of Gongshan. Even though there are some little transformations, yet these disparities are not a sige for a level of species, such as the tubercles and posterior of temporal fold in size, which increase accompanying with body size.