鸡Z染色体上DMRT1基因的多重跨染色体剪接
Interchromosomal Trans-Splicing of DMRT1 Gene on Chicken Chromosome Z
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摘要: 性别决定与分化发育是同时涉及生命现象中两种细胞分裂(有丝分裂和减数分裂)形式的惟一的分化发育过程。对该过程中关键基因DMRT1的转录分析，发现位于鸡Z染色体上的DMRT1基因分别同时与4号染色体上的CENP C1基因、5号染色体上CD5R基因和2号染色体上37LRP/p40基因发生跨染色体剪接，由此构成了新的跨染色体剪接本DMRT1-CENP C1、DMRT1-CD5R和DMRT1-37LRP/p40。对其剪接位点的分析，发现两段染色体序列存在的重叠区可能在这种剪接中起着重要作用。DMRT1基因在转录过程中同时与多个染色体上基因发生多次跨染色体剪接的发现，无疑有助于对在转录水平上的多样性基因调控以及性别决定与分化发育等的认识开辟另一新途径。Abstract: Sex determination/differentiation is the only developmental process involving two types of cell divisions (mitosis and meiosis). Analysis of transcription of key gene DMRT1 in the process showed that DMRT1 gene on the chromosome Z was trans-spliced with CENP C1 on the chromosome 4, CD5R on the chromosome 5 and 37LRP/p40 on the chromosome 2, which composed a rare form of transcripts DMRT1-CENP C1, DMRT1-CD5R and DMRT1-37LRP/p40 respectively. Overlapping regions between splicing sites on both chromosomes were observed, which would play an important role in the interchromosomal trans-splicing. The findings that multiple genes on different chromosomes are trans-spliced simultaneously with the DMRT1 during transcription undoubtedly help in understanding diversity of gene regulations at transcriptional level and sex determination and differentiation mechanisms.
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