Abstract: Testicular microstructure was investigated in 38 stream salamanders (Batrachuperus tibetanus) collected at the north slope of Qinling Mountain from Jan.to Dec.of 2001 and 2003.The results showed that spermatogenesis in this species followed an annual cycle,was the discontinuous type.Proliferation of spermatogonia was divided into two stages,one was from Sep.to Nov.,and the other was from Apr.to May next year.Meiosis occurs in late Jun.-Jul.,and the spermiogenesis began late Jul.and spermatozoa were present in Aug.Each lobule was distinguished from the immature region and mature region.The immature region served as a reservoir of spermatogonia,which can mitose into a proliferative region.The proliferative region transformed into the mature region,which served as the spermiogenesis site and store sperm.After spermiation,the mature region changed into the evacuated region,which was substituted by the proliferative region progressively.Spermatogenic progress was synchronized in all testicular lobules,and no maturational wave was discernible in the testis.