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陈云鹤, 周星良, 芍世康, 乔以炯. 1983: 前环藻(Amphidinium carterae)(涡鞭毛虫)染色质碱性蛋白的研究. 动物学研究, 4(4): 315-320.
引用本文: 陈云鹤, 周星良, 芍世康, 乔以炯. 1983: 前环藻(Amphidinium carterae)(涡鞭毛虫)染色质碱性蛋白的研究. 动物学研究, 4(4): 315-320.
CHEN Yun-he, ZHOU Xing-liang, GOU Shi-kang, LI Jing-yan. 1983. Biochemical Study of Chromosomal Basic Protein From the Dinoflagellate Amphydinium carterae. Zoological Research, 4(4): 315-320.
Citation: CHEN Yun-he, ZHOU Xing-liang, GOU Shi-kang, LI Jing-yan. 1983. Biochemical Study of Chromosomal Basic Protein From the Dinoflagellate Amphydinium carterae. Zoological Research, 4(4): 315-320.

前环藻(Amphidinium carterae)(涡鞭毛虫)染色质碱性蛋白的研究

Biochemical Study of Chromosomal Basic Protein From the Dinoflagellate Amphydinium carterae

  • 摘要: 本研究用甲醇固定、细胞匀浆、0.3N HCI抽提及丙酮沉降的四步法提取了属于典型涡鞭毛虫类的前环藻(Amphidinium carterae)之染色质碱性蛋白及作为对照的小牛胸腺组蛋白,并以酸尿素系统的聚丙烯酰胺凝胶电泳,对所提取的蛋白作了对比检查。结果小牛胸腺的总组蛋白被分离成H1、H3、H2A、H2B及H4五条电泳带;前环藻的蛋白样品在组蛋白电泳区唯一出现了一条电泳带,其电泳迁移率相当于小牛胸腺组蛋白H4。根据本结果和另外一些作者对其他一些涡鞭毛虫类的生化和细胞化学研究的结果,表明以往主要根据经典的细胞化学研究之结果而认为涡鞭毛虫类的细胞核或染色体不含组蛋白或碱性蛋白作为其一重要特征,是并不全面和可靠的。包括本研究在内的儿个生化研究结果也暗示了涡鞭毛虫类的染色质主要含一种电泳迁移率类似于组蛋白H4的碱性蛋白可能是一普遍现象。

     

    Abstract: We examined the chromosomal basic protein from the Dinoflagellate Amphidinium carterae.The chromosomal basic protein of A.carterae and calf thymus histones as control were isolated by flur steps:Methanol-fixation,cell-homogenization,0.3N HCI-extraction and acetone-sedimentation.The qualitative examination of the isolated proteins was made in acid urea polyacrylamide gel electrophoretic system.As a result,the histones from calf thymus were separated into five bands:H1,H3,H2A,H2B and H4,and no other protein bands were observed.The extracted protein from A.carterae showed only one single band.Its electrophoretic mobility was similar to that of calf thymus histone H4.This result together with those of other authors on biochemical and cytochemical studies all indicates that the chromosomes of dinoflagellate contain chromosomal basic protein.The opinion that the lack of chromosomal basic proteins is an important character of dinoflagellate seems to be incorrect.

     

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