Abstract: A method of collect venom from the spider (Selenocosmia huwena) was reported.The biological activities of the crude venom were analyzed.The LD50 of the venom in mice and cockroach were 1.16 mg/kg and 300 μg/g respectively.It has enzyme activities of hyaluronidase,alkaline phosphatase,protease.and DNAse,but no choline esterase and phospholipase A were found in it.In the isolated toad sciatic nerve-sartorius preparation the venom at a concentration of 3 mg/mL was found to block the neuromuscular transmission irreversibly.In mice,the crude venom in dosage of 5 mg/kg caused respiratory failure in about 20 seconds.