Abstract: 12 donors (3-9 years old dairy cows) were superovulated with FSH-p twice daily in the dose of 36 mg for four days in 10-12th days of cycle after synchronized with PGF2α.At the third day of superovulation,additional 35 mg PGF2α were administrated intramuscularly.A total of 135 embryos were recovered 6-7 days after insermination.Of these embryos,87.47% (118/135) showed good quality (average 9.8 embryos per donor).Particularly,we got 76 embryos from 3 donors (24,26 and 27 respectively) and 69 of 76 were good quality (23 embryos per donor).After transferred to synchronized recipients (20 dairy cows and 20 cattle) with total 40 embryos.7 dairy cows (35%) and 8 cattle (40%) were pregnant.The average pregnant rate were 37.5%.A total of 13 heifers were delivered to birth from 7 dairy cow and 6 cattle in this experiment.