Abstract: The karyotypes of 20 species of 8 families of Climber birds were studied from bone marrow cells with colchicine-hypotonic-airdrying technique.The results were given as follow:Picidae:Jynx torquilla,2n=90;Dendrolopos hyperythrus,2n=92;D.leucotos,2n=90;D.kizuki,2n=92;Picus canus,2n=92.Upupidae:Upupa epops,2n=126.Alcedinidae:Alcedo atthis,2n=132;Halcyon pileata,2n=84.Cuculidae:Cuculis canorus,2n=78;C.saturatus,2n=76;C.microptaus,2n=78;C.fugax,2n=78.Apodidae:Apus pacificus,2n=62;Hirumd-apus candacutus,2n=64.Psittacidae:Melopsittacus undulatus,2n=62.Caprimulgidae:Caprimulgus indicus,2n=76.Strigidae:Otus scops,2n=76;O.bakkamoena,2n=82;Asio flammeus,2n=82;Athene noctus,2n=80.The 15 families of Climber birds have usually been divided into 3 foottypes:Zygodactylous,Syndatylous and Heterodactylous.Sibley and Ahlquist (1988) assigned them to 3 Parv-classes:Picae,Coraciae and Passerae.These two divisions are very different each other.The karyotype comparisons of the Climber birds support the division by Sibley et al.,according DNA-DNA hybridization data.