小鼠淋巴结及胸腺T、B淋巴细胞的鉴别

Identification of Mouse Lymph Node and Thymus T,B Lymphocyte

摘要: 本文用细胞化学ACP偶氮染色法和5 N“Gomori”染色法鉴别小鼠淋巴结及胸腺T、B淋巴细胞。测定小鼠上述淋巴组织细胞悬液样品中ACP及5 N活力所得的结果：淋巴结的ACP阳性细胞为67%左右，5 N阳性细胞为31%左右，胸腺的ACP阳性细胞为97%，5 N阳性细胞为10%。上述结果是相互符合的，也与目前已报导的小鼠及人的淋巴结和胸腺T、B淋巴细胞百分数基本符合。

Abstract: The azo dye method for acid phosphatase and "Gomori" method for 5 Nucleotidase were used to identify the T,and B lymphocytes of mouse lymph node and thymus cytochemically.By employing these methods,the estimation of ACP and 5 N activities of parallel samples from the lymph node and thymus cell suspensions were carried out in order to verify the ACP and 5N positive lymphocyte percentages obtained previously.The ACP and 5 N positive lymphocytes thus estimated for lymph node were found to be 67% and 31%,whereas those for the thymus were around 97% and 10% respectively.Above mentioned results were not only well fitted with each other but also in good accordance with those already published.