Abstract: A whole blood microculture method for obtaining metaphase chromosome and a technique of Trypsin-Giemsa banding were used in this study.T-G banding chromosomes of man are compared with those of golden monkey and rhesus monkey.Three cells of each species were analysed.The results show that the banding pattern of each pair of homologous chromosomes in different cells of the same species is analogy,but for cells in different species the banding pattern of most chromosomes is partly similar and that of only a few chromosomes is almost completely analogy.