碘标神经毒素——乙酰胆硷受体复合物对重症肌无力研究的初步应用

Primary Use of Iodine Labeled Neurotoxin-Acetylcholine Receptor Complex in Myasthenia Gravis Research

摘要: 眼镜蛇神经毒素化学性质稳定，易于保存，用131Ⅰ或125Ⅰ标记，方法简便，比活性高，能专一性地与菸硷型乙酰胆硷受体结合，常作为一种分子探测剂，用来研究蛇毒的毒理作用、测定菸硷型乙酰胆硷受体的浓度，于临床还可以检测重症肌无力患都抗乙酰胆硷受体抗体。对研究神经肌接头的生理功能，阐明重症肌无力的发病机理、辅助诊断和观察疗效均有实际意义。

Abstract: By means of chloramine T oxidation method,α-neurotoxin from Naja naja was labeled with 131Ⅰ.After passing over a sephadex G-25 column and applied assending paper chromatograph,the rate of label reached to higher degree (80%).The specific activity was 6 μCi/μg.With the help of two dimentional double immunodiffusion and rocket immunoelectrophotesis self-illumination,its antigenicity still maintained was proved.