Abstract: This work used Immunohistochemistry to examine the expression of myelin basic protein and accumulation of oligodendrocytes in Pchdaknockout and control littermate mice. Data showed that in Pchda knockout mice, Myelin proteins decrease in the central nervous systemand mature oligodendrocytes in the cerebellum also decrease. Furthermore, deletion of the Pcdha cluster does not cause any change to the axons and astrocytes in quantification of relative marker proteins. These findings suggest that the Pcdha cluster may be required for myelination and oligodendrite development of the brain in mice, and that Pcdha cluster may play a key role in the development of the central nervous system.