Protocadherin x gene cluster is required for myelination and oligodendrocyte development
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摘要: 该文通过免疫组化及蛋白免疫印迹的方法分别对Pcdha基因敲除和对照组小鼠的中枢神经系统内的髓鞘碱性蛋白表达以及少突胶质细胞的发育进行了测定。结果表明：1) Pcdha基因缺失小鼠中枢神经系统中的髓鞘碱性蛋白较对照组小鼠明显减少；2) Pcdha基因敲除可导致少突胶质细胞发育异常：在小脑中, 处于成熟期的少突胶质细胞减少, 而处于前体细胞阶段的少突胶质细胞增多。上述结果提示Pcdha可以通过调控少突胶质细胞的成熟过程进而影响髓鞘的形成。Abstract: This work used Immunohistochemistry to examine the expression of myelin basic protein and accumulation of oligodendrocytes in Pchdaknockout and control littermate mice. Data showed that in Pchda knockout mice, Myelin proteins decrease in the central nervous systemand mature oligodendrocytes in the cerebellum also decrease. Furthermore, deletion of the Pcdha cluster does not cause any change to the axons and astrocytes in quantification of relative marker proteins. These findings suggest that the Pcdha cluster may be required for myelination and oligodendrite development of the brain in mice, and that Pcdha cluster may play a key role in the development of the central nervous system.
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Key words:
- Protocadherin /
- Myelin /
- Oligodendrocyte /
- Development
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