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Phenotype analysis and mutant gene location of ventral yellow mouse (VYSlac)

SHI Mei-Lian XU Ping YIN Xiao-Shu YANG Wei-Wei GU Mei-Er YU LI-Ping LIU Gui-Jie WU Bao-Jin

SHI Mei-Lian, XU Ping, YIN Xiao-Shu, YANG Wei-Wei, GU Mei-Er, YU LI-Ping, LIU Gui-Jie, WU Bao-Jin. Phenotype analysis and mutant gene location of ventral yellow mouse (VYSlac). Zoological Research, 2012, 33(3): 290-297. doi: 10.3724/SP.J.1141.2012.03290
Citation: SHI Mei-Lian, XU Ping, YIN Xiao-Shu, YANG Wei-Wei, GU Mei-Er, YU LI-Ping, LIU Gui-Jie, WU Bao-Jin. Phenotype analysis and mutant gene location of ventral yellow mouse (VYSlac). Zoological Research, 2012, 33(3): 290-297. doi: 10.3724/SP.J.1141.2012.03290

腹侧黄斑小鼠的部分特征及其突变基因的染色体定位

doi: 10.3724/SP.J.1141.2012.03290
基金项目: 上海市科学技术委员会科研计划项目(10140902700);国家自然科学基金项目(31071092)
腹侧黄色被毛小鼠和B6小鼠血液生理指标
补充材料二
详细信息
  • 中图分类号: Q959.837;R-332;Q344;Q954.4

Phenotype analysis and mutant gene location of ventral yellow mouse (VYSlac)

  • 摘要: 腹侧黄斑小鼠(VYSlac)是在B6小鼠繁殖生产过程中被发现、分离的突变系小鼠,呈单基因显性遗传,其头颈及躯干的腹侧为黄色。VYSlac腹部表皮多巴(+)黑色素细胞及毛囊内黑色素较其背景品系B6少;腹部毛发颜色浅黄、多数无黑色素沉积,但结构正常。通过微卫星标记与48只F2小鼠(VYSlacD2F1回交D2)的连锁分析发现,突变基因与D2Mit229间的LOD值为5.79,确定连锁。随后,在突变基因附近反复多次筛选新的微卫星或SNP标记,通过对196例F2小鼠的多次连锁分析,将突变基因所在区域缩小到rs13476833(距着丝粒149804749bp)与rs27310903(距着丝粒155060764bp)间约5256015bp的范围内。
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出版历程
  • 收稿日期:  2011-11-21
  • 修回日期:  2012-04-09
  • 刊出日期:  2012-06-22

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