Cloning of hemoglobin-α1 from half-smooth tongue sole (Cynoglossus semilaevis) and its expression under short-term hypoxia
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摘要: 该文克隆了海洋鱼类——半滑舌鳎（Cynoglossussemilaevis,Cs）的血红蛋白α1（CsHb-α1）基因全长cDNA序列，并研究了其在短期低氧胁迫下的表达变化。CsHb-α1cDNA全长594bp，编码144个氨基酸。氨基酸序列分析表明，该基因与其他物种的Hb具有较高的序列相似性。实时定量PCR检测显示CsHB-α1在心、肝、脾脏、肾脏和血液中表达量较高。与对照组相比（溶解氧：6.2mg/L），在短期（5～120min）及36h低氧胁迫（溶解氧：1.0mg/L）后，CsHb-α1在心、肝脏、脾脏、肾脏、血液和腮腺中表达量明显升高。这表明Hb-α1的上调是半滑舌鳎适应低氧胁迫的一个重要分子组成。
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关键词:
- Cynoglossus semilaevis /
- Hemoglobin /
- Gene clone /
- Hypoxia stress
Abstract: This study cloned the hemoglobin α1 from the marine teleost, the half-smooth tongue sole (Cynoglossus semilaevis), and then examined its expression under hypoxia exposure. The full-length of CsHb-α1 (594 bp) cDNA contains an open reading frame encoding 144 amino acids. Sequence analysis shows that the predicted CsHb-α1 amino acids shares high identities with that of other species. Real-time PCR showed that CsHb-α1 was highly expressed in the heart, liver, spleen, kidney and blood. Five to 120 min esposure and long-term (36 h) exposure to hypoxia (1.0 mg/L) significantly increased CsHb-α1 mRNA expression in most tissues compared to those fish held in normoxic conditions (dissolved oxygen (DO): 6.2 mg/L). These results suggested that the up-regulation of Hb-α1 is an important component for adaptation of half-smooth tongue sole to short-term hypoxia.-
Key words:
- Cynoglossus semilaevis /
- Hemoglobin /
- Gene clone /
- Hypoxia stress
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