虎纹捕鸟蛛毒素-Ⅵ的分离纯化与鉴定

Purification and Identification of Huwentoxin-Ⅵ,a Neurotoxic Peptide from the Venom of Selenocosmia huwena

摘要: 通过离子交换及反相HPLC，从虎纹捕鸟蛛毒液中分离得到1种新的哺乳类神经毒素，命名为虎纹捕鸟蛛毒素-Ⅵ(Huwentoxin-Ⅵ，HWTX-Ⅵ)。MALDI-TOF质谱仪分析及氨基酸序列分析表明该多肽毒素分子量为4.440 18 kDa。序列为H2N-CIGEG VPCDE NDPRC CSGLV VLKKT LHGIW IKSSY CYKCK-COOH。其中6个Cys形成3对二硫键。小鼠脑内注射实验表明，HWTX-Ⅵ对神经系统具有明显的致瘫作用，这种致瘫作用又具有可逆性。

Abstract: A novel mammal-specific neuron toxin,Huwentoxin-Ⅵ(HWTX-Ⅵ),was purified from the venom of the spider Selenocosmia huwena,by ion exchange chromatography and reversed phase HPLC.The molecular weight of the toxin is 4.440 18 kDa determined by the analysis of MALDI-TOF mass spectrum and the sequence is:H2N-CIGEG VPCDE NDPRC CSGLV VLKKT LHGIW IKSSY CYKCK-COOH determined by the amino sequence analysis,in which six Cys residues form three pairs of disulfide bridge.Rat brain injection experiments show that Huwentoxin-Ⅵ has obvious paralysis effect on neuron system and the paralysis effect has reversibility.