Abstract: Fifteen restriction endonucleases,Bam HⅠ,BglⅡ,DraⅠ,Eco RⅠ,Eco RⅤ,Hin dⅢ,KpnⅠ,PstⅠ,PvuⅡ,SacⅠ,SalⅠ,ScaⅠ,StuⅠ,XbaⅠ,and XhoⅠ were used to investigate the mitochondrial DNA polymorphism of Guizhou native cattle and Guizhou black and white cow.Two types of mtDNA molecules were detected in Guizhou native cattle:one showed the type of yellow cattle (Bos taurus),the other showed that of zebu cattle (Bos indicus),suggesting their European and zebu origins respectively.The mtDNA in Guizhou black and white cow also consists of two restriction types.We also discussed the nomenclature of "Panjiang cattle"and "Wuling cattle".