Abstract: Anopheles minimus is one of main vectors of malaria in southeast Asia.Recent studies have been focused on morphology,ecology,and contagion of plasmodiums.In this paper,random amplified polymorphic DNA markers (RAPDs) were used to estimate genetic relationships of 29 Anopheles minimus from 5 geographic populations in Yunnan,China.Among 29 individuals,four were males.For twelve arbitrary primers analyzed,two of them gengrates monophic loci.Out of a total of 57 RAPD bands scored,37 were varied.Molecular phylogenetic tree derived from genetic distance suggested that the RAPDs were more related to geographic condition than to sexual distinction,There were genetic divergences among different geographic populations.Furthermore,it was implied that RAPD was one of prospering genetic markers for studying taxonomy and evolution of Anopheles minimus.