Abstract: The secondary-structural features of TATA box is important for the initial process of RNA transcription in eukaryotic.After observing the secondary-structures of 142 genes promotor sequences,we proposed this structural model of the stems and loops for the nucleotide sequences of the TATA box in eukaryotic.Our results suggested that the structural features of TATA box in structural models of the stems and loops were closely associate with the recognition and interaction of the transcription factor and RNA polymerase.We got a consistent result by comparing with the corresponded secondary-structural features of E.coli.Among the distribution of hairpin,interloop,and single strand there was a difference.